葡萄离体培养及快速繁殖

取无核白鸡心 美人指 甬优一号 等3 个葡萄品种0.5 cm 长的单芽嫩茎作为外植体, 接种于不同配方的培养基上。结果表明 无核白鸡心 美人指 接种于GS 分别附加6-BA 1.0 mgL-1和2.0 mgL-1的培养基, 甬优一号 接种MS 附加6-BA 1.0 mgL-1培养基诱导芽, 芽的诱导率分别达100 %, 100 %和86 %。当芽伸长至1.0 ～ 1.5 cm 时, 3 个品种均转接于MS附加IAA 0.05 mgL-1 , 6-BA 1.0 mgL-1和GA3 2.0mgL-1的分化培养基, 芽的分化最佳, 分化率达80 %～ 86 %, 增殖4.2 倍左右, 苗生长健壮。将无核白鸡心 转入1/2MS 附加IBA 1.0 mgL-1 , 美人指 甬优一号 转入1/2MS 附加IBA 2.0 mgL-1的培养基, 生根率达84 %～ 96 %, 平均根数在5 ～ 7 条之间, 根生长良好。表6 参10

Tissue culture and propagation in grape

Taking the stems of 0.5 cm with single bud as the explants to the different culturemediums , the results showed that the rates of differentiation of buds that were the highest in all treatments were 100 % for cv . Wuhebaijixin , and cv .Meirenzhi and 86 % for cv .Yongyou F1 on the GS medium containing 6-BA 1.0 mgL-1 or 6-BA 2.0 mgL-1 .When the buds grew 1.0 ～ 1.5cm , they were transferred to the MS medium supplemented with IAA 0.05 mgL-1 or 6-BA 1.0mgL-1 orGA 2.0 mgL-1 with the differentiation rate of 80 % ～ 86 % and the proliferation multiple of 4 for this three varieties and the shoots grew vigor , which were the most favourite medium for the induction and differention of buds in this test .If the grape of Wuhebaijixin was changed into the 1/2 MS medium containing IBA 1.0 mgL-1 and Meirenzhi and Yongyou F1 were changed into 1/2 MS medium containing IBA 2.0 mgL-1 , the shoots developed nice roots with rooting rate of 84 %～ 96 % and rooting number of 5 ～ 7 .These media were the most significant for roting in all treatments .