| 黑荆树茎尖培养技术的研究 |
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| 引用本文： | 张华通 翟应昌. 黑荆树茎尖培养技术的研究[J]. 广东林业科技, 1996, 12(4): 17-22 |
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| 作者姓名： | 张华通 翟应昌 |
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| 作者单位： | 广东省林业科学研究所 |
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| 摘 要： | 以黑荆树无菌种子苗的茎类为材料，研究了用不同的细胞分裂素，生长的浓度及其配比，培养基成分的浓度，对茎尖诱导分化芽，芽的伸长及生根的影响，研究结果；培养基为ＭＳ＋６－ＢＡ６．０ｍｇ／Ｌ＋ＬＢＡ０．０１ｍｇ／Ｌ＋ＧＡ３０．１ｍｇ／Ｌ有利于促进茎尖丛生茎的形成和伸长，１／２ＭＳ＋ＮＡＡ０．６ｍｇ／Ｌ有利于诱导芽生根，试管苗移栽成活率达９０％，并在温室里生长正常。
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| 关 键 词： | 黑荆树 茎尖培养 诱导生根 移栽 |
MICROPROPAGATION OF SHOOT TIP FROM SEEDLING OF BLACK WATTLE |
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| Zhang Huatong Zhai Yingchang Zhou Zhijian. MICROPROPAGATION OF SHOOT TIP FROM SEEDLING OF BLACK WATTLE[J]. Forestry Science and Technology of Guangdong Province, 1996, 12(4): 17-22 |
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| Authors: | Zhang Huatong Zhai Yingchang Zhou Zhijian |
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| Abstract: | Shoot tips (5 mm long) from seedlings of Acacia mearnsii in vitro as explants were grown on different media supplemented with different concentrations and compounds of auxetic and auxin. The results showed that MS+6 BA 6.0 mg/L+IBA 0.01 mg/L+GA 3 0.1 mg/L was most favourable for shoot formating and elongating, 1/2MS+NAA 0.6 mg/L was most favourable for shoot rooting. The survival rate was up to 90% after transplanting in soil. |
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| Keywords: | black wattle Shoot tip culture medium |
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