不同季节肉用种公牛精液品质与生理常值、血清及精清生化指标相关关系研究

以利木赞和夏洛来种公牛为试验牛，研究不同季节对其精液品质、冻精产量、生理常值、血清及精清生化指标的影响。结果表明：（1）与其它季节相比，夏季肉用种公牛的原精活力、冻精活力、活精于百分数、顶体完整率均显著降低，而精于畸形率显著升高（P〈0．05）。精子密度以秋季最低。（2）冻精产量夏季处于最低水平，平均每月仅为7388支。（3）夏季肉用种公牛的血清LH、睾酮、T3、皮质醇的含量分别为48．12、4．15、1．11、4．89ng／L，显著低于春、秋、冬季（P〈0．01）。（4）夏季肉用种公牛血清钠、钾、钙、镁均处于最低水平，显著低于其它季节（P〈0．01）。（5）夏季肉用种公牛精清睾酮水平明显下降，显著低于春季、秋季和冬季。（6）精清钠、钾、钙夏季含量最低，分别为83．12mmol／L、18，77mmol／L和4．63mmol／L；精清镁含量由春季至秋季呈显著下降趋势；精清磷含量夏季最高，为4．65mmol／L，显著高于春季、秋季和冬季。（7）肉用种公牛精清ALP夏季含量为1784．3U／L，显著高于春、秋两季，但低于冬季。（8）精清睾酮与精液品质各指标存在显著相关；血清生化指标与精清生化指标存在显著相关；直肠温度、呼吸频率与精液品质及血清生化指标存在显著相关性，与精清生化指标相关性不大。

Research on the Correlation of Seminal Quality, Physiological Response, Blood Serum Biochemical Parameters and Seminal Plasma Biochemical Indexs of Beef Breeding Bulls in Different Seasons

Limousin bulls and Charolais bulls were used in the present study to examine the influence of different seasons on semen quality,frozen semen productivity,physiological response,blood biochemical parameters and seminal plasma biochemical parameters.Results showed that:(1)The sperm motility,frozen sperm motility,percentage of motile sperm and percentage of intact acrosome were significantly lower,and the rate of abnormal sperm is significantly higher in summer than that of other seasons(P<0.05),while sperm concentration was at the lowest level in autumn.(2)The quantity of frozen semen production in summer was at the lowest level,only(7 388) count each month.(3)The mean concentrations of blood serum LH(48.12 ng/L),testosterone(4.15 ng/L),T_3(1.11 ng/L) and cortisol(4.89 ng/L) were lower in summer than that of other seasons(P<0.01).(4)Compared with other seasons,the mean levels of blood serum sodium,potassium,calcium and magnesium decreased significantly in summer(P<0.01).(5) The concentration of seminal plasma testosterone of beef breeding bull was lower in summer than that of spring,autumn and winter significantly(P<0.05).(6) the mean concentrations of seminal plasma sodium(83.12 mmol/L),potassium(18.77 mmol/L) and calcium(4.63 mmol/L) were at the lowest level in summer compared with that of spring,autumn and winter;the concentration of magnesium in seminal plasma had the significant drop trend from spring to autumn,but the mean concentrations of seminal plasma phosphorus(4.65 mmol/L) in summer was on the highest level and significantly higher than that of other seasons(P<0.01).(7) In summer,the mean concentration of seminal plasma ALP(1 784.3 U/L) was higher than that of spring and autumn(P<0.05),but lower than that of winter(P<0.01).(8) Seminal plasma testosterone was found to be significantly correlated with the parameters of seminal quality(P<0.05).The serum biochemical parameters were significantly correlated with seminal plasma biochemical parameters(P<0.05).The respiratory rate and rectal temperature were found to be significantly correlated with the parameters of seminal quality and serum,but there was no significant correlation with seminal plasma biochemical parameters(P<0.05).