外源NO对干旱下刺槐幼苗抗氧化特性的影响

采用盆栽试验,研究了外源一氧化氮(NO)处理对干旱条件下刺槐幼苗抗氧化特性的影响。结果表明,与正常水分(田间持水量的75%～80%)处理相比,干旱胁迫(田间持水量的45%～55%)处理显著提高了刺槐幼苗叶片的超氧化物歧化酶(SOD)和过氧化物酶(POD)活性、还原型抗坏血酸(AsA)和还原型谷胱甘肽(GSH)含量、相对电导率和丙二醛(MDA)含量,增幅分别为33.3%、175%、31.4%、38%、150%和101.6%。与干旱胁迫处理相比,100μmol/L硝普钠(SNP)+干旱处理显著提高了刺槐幼苗叶片的SOD和POD活性、AsA和GSH含量,增幅分别为20%、81.8%、19.6%、27.5%,显著降低了相对电导率和MDA含量,降幅分别为32%、27.2%。这表明,外源NO处理可以缓解干旱胁迫对刺槐幼苗所造成的伤害。

Effects of Exogenous Nitric Oxide on Antioxidant Properties of Robinia pseudoacacia L.Seedling Leaves under Drought Stress

This paper dealt with effects of exogenous nitric oxide on the antioxidant properties of Robinia pseudoacacia L.leaves under drought stress.The results showed that drought stress(45%-55% field capacity) significantly increased the activities of SOD(33.3%) and POD(175%),the contents of AsA(31.4%) and GSH(38%),and the permeability of plasma membrane(150%) and MDA content(101.6%),compared with normal water treatment(75%-80% field capacity).The treatment of 100 μmol/L SNP and drought significantly increased the activities of SOD(20%) and POD(81.8%),and the contents of AsA(19.6%) and GSH(27.5%),and significantly decreased the permeability of plasma membrane(32%) and MDA content(27.2%).In conclusion,exogenous nitric oxide could alleviate the injury caused by drought stress.