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应用相互嫁接技术研究棉花对黄萎病的抗性
引用本文:胡雨薇,郝俊杰,贾新合,马奇祥,崔小伟,高俊山,李宾,郭利娟,王庆东.应用相互嫁接技术研究棉花对黄萎病的抗性[J].河南农业科学,2012,41(11):88-91.
作者姓名:胡雨薇  郝俊杰  贾新合  马奇祥  崔小伟  高俊山  李宾  郭利娟  王庆东
作者单位:1. 郑州大学生物工程系,河南郑州450001;河南省农业科学院植物保护研究所,农业部华北南部农作物有害生物综合治理重点实验室/河南省农作物病虫害防治重点实验室,河南郑州450002;新乡医学院三全学院,河南新乡453003
2. 河南省农业科学院植物保护研究所,农业部华北南部农作物有害生物综合治理重点实验室/河南省农作物病虫害防治重点实验室,河南郑州450002
3. 郑州市农林科学研究所,河南郑州,450005
4. 开封市农林科学研究院,河南开封,475004
5. 禹州市农业林业局,河南禹州,461670
6. 郑州大学生物工程系,河南郑州,450001
基金项目:国家转基因生物新品种培育重大专项,国家棉花现代农业产业技术体系项目
摘    要:选用对棉花黄萎病表现不同抗性的棉花材料,应用相互嫁接的方法将抗病和感病棉花品种组合在一起构建嫁接系统,将其种植在连作多年的棉田,调查棉花黄萎病的发生情况。结果表明,自身嫁接苗与自根苗抗性差异不显著;相互嫁接植株抗/感(接穗/砧木,下同)和感/抗组合抗病效果明显强于感/感组合,但较抗/抗组合抗病性下降。研究认为,抗病材料无论作砧木还是接穗,均能抑制黄萎病的发生,说明抗病材料植株整体对棉花黄萎病菌具有抵抗能力。

关 键 词:棉花  黄萎病  相互嫁接  抗性

Use of Reciprocal Grafting to Study Verticillium Wilt Resistance of Cotton
HU Yu-wei , HAO Jun-jie , JIA Xin-he , MA Qi-xiang , CUI Xiao-wei , GAO Jun-shan , LI Bin , GUO Li-juan , WANG Qing-dong.Use of Reciprocal Grafting to Study Verticillium Wilt Resistance of Cotton[J].Journal of Henan Agricultural Sciences,2012,41(11):88-91.
Authors:HU Yu-wei  HAO Jun-jie  JIA Xin-he  MA Qi-xiang  CUI Xiao-wei  GAO Jun-shan  LI Bin  GUO Li-juan  WANG Qing-dong
Institution:1*(1.Bioengineering Department of Zhengzhou University,Zhengzhou 450001,China; 2.Key Laboratory for IPM of Crops in Southern North China,Ministry of Agriculture/Henan Key Laboratory for Crop Pest Control,Institute of Plant Protection,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China; 3.Sanquan Medical College of Xinxiang Medical University,Xinxiang 453003,China; 4.Zhengzhou Institute of Agriculture and Forestry Sciences,Zhengzhou 450005,China; 5.Kaifeng Academy of Agriculture and Forestry Sciences,Kaifeng 475004,China; 6.Yuzhou Administration of Agriculture and Forestry,Yuzhou 461670,China)
Abstract:In an attempt to better understand the resistance mechanism of cotton to Verticillium wilt,cotton lines with different resistance levels to Verticillium wilt were selected,and reciprocal grafting were made between resistant and susceptible genotypes.The grafted combinations were planted in the naturally infected cotton field,and the data of cotton Verticillium wilt were investigated.The results indicated that the resistance level was not significantly different between self-root cotton and their self-grafted cotton.The resistance of resistant/susceptible and susceptible/resistant grafted combinations was higher than that of susceptible/susceptible combination,but lower than that of resistant/resistant combination.Resistant cotton genotypes either as stock or scion can inhibit Verticillium wilt,which indicates that the whole plant of resistant cotton can resist to Verticillium wilt.
Keywords:cotton  Verticillium wilt  reciprocal grafting  resistance
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