| 苯磺隆对鲫鱼肝细胞内膜系统形态和转氨酶活性的影响 |
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| 引用本文： | 张彬彬,王彦美,王玉芳,付娟. 苯磺隆对鲫鱼肝细胞内膜系统形态和转氨酶活性的影响[J]. 河南农业科学, 2012, 41(10): 145-147,157 |
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| 作者姓名： | 张彬彬 王彦美 王玉芳 付娟 |
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| 作者单位： | 1. 滨州学院生命科学系,山东滨州256603;滨州市食品安全重点实验室,山东滨州256603
2. 滨州学院生命科学系,山东滨州,256603 |
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| 基金项目： | 滨州市科技发展计划项目 |
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| 摘 要： | 为揭示苯磺隆对水生生物细胞生物标志物——内膜系统和转氨酶〔谷丙转氨酶(GPT)、谷草转氨酶(GOT)〕的影响,采用等比数列设计质量浓度梯度对鲫鱼进行慢性中毒试验。结果表明:随苯磺隆质量浓度的增加,鲫鱼肝细胞内膜系统中线粒体肿胀及空泡化,内质网空泡化,异噬型溶酶体增多。0.64～5.07mg/L的苯磺隆处理24h、48h、96h,均使上述2种转氨酶活性增强,96h、10.14mg/L苯磺隆处理组GPT活性明显低于同等质量浓度下48h处理组,提示苯磺隆对水生生物标志物具有一定的毒性影响。
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| 关 键 词： | 苯磺隆 鲫鱼 肝脏 内膜系统 谷丙转氨酶 谷草转氨酶 |
Effects of Tribenuron-methyl on Hepatic Endomembrane System and Aminotransferase of Carassius auratus |
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| ZHANG Bin-bin , WANG Yan-mei , WANG Yu-fang , FU Juan. Effects of Tribenuron-methyl on Hepatic Endomembrane System and Aminotransferase of Carassius auratus[J]. Journal of Henan Agricultural Sciences, 2012, 41(10): 145-147,157 |
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| Authors: | ZHANG Bin-bin WANG Yan-mei WANG Yu-fang FU Juan |
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| Affiliation: | 1(1.Life Science Department of Binzhou University,Binzhou 256603,China; 2.Key Laboratory for Food Safety of Binzhou,Binzhou 256603,China) |
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| Abstract: | To study the effects of tribenuron-methyl on biomarkers of aquatic organism cells in the intima and on two aminotransferase,GPT and GOT,a chronic poisoning test was performed by geometric sequence design concentration gradient and the experiment data was analyzed by factorial design variance.The results showed that with concentration elevated,mitochondrion swelled and vacuolated,ER vacuolated,heterolysosome grew in the hepatic endomembrane system.Treatment of tribenuron fluid lower of 0.64-5.07 mg/L caused the enhancement of two transaminase activity.However,the group of high concentration of 10.14 mg/L decreased their activities after 96 h,indicating that tribenuron-methyl has toxicity impact on aquatic biomarkers. |
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| Keywords: | tribenuron-methyl Carassius auratus hepar endomembrane system GPT GOT |
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