| 小麦抗叶锈病基因近等基因系TcLr35基因表达差异研究 |
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| 引用本文： | 陈云芳,杨文香,闫红飞,刘力强,褚栋,刘大群.小麦抗叶锈病基因近等基因系TcLr35基因表达差异研究[J].植物病理学报,2007,37(1):109-112. |
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| 作者姓名： | 陈云芳 杨文香 闫红飞 刘力强 褚栋 刘大群 |
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| 作者单位： | 河北农业大学植物保护学院河北省农作物病虫害生物防治工程技术研究中心, 保定 071001 |
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| 摘 要： | cDNA—AFLP技术结合了RT-PCR和AFLP技术，用于分析植物mRNA的表达差异。该技术保留了AFLP技术的可靠性与高效性，可广泛地应用于植物生长发育过程基因分离与表达特性的研究。小麦叶锈病是世界上影响小麦产量的重要病害之一，提高品种抗病性是防治该病害最经济有效的方法。在目前已发现的近90个抗叶锈基因中，有56个基因已被正式定名，其中有9个成株抗性基因和慢锈性基因，即Lr12、Lr13、Lr22a、Lr22b、Lr34、Lr35、Lr37、Lr48和Lr49。Lr35基因来源于拟斯卑尔托山羊草（Aegilops speltoides），该基因位于2B染色体上，是一个十分有效的成株抗性基因。
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| 关 键 词： | 基因表达差异 小麦产量 抗叶锈病基因 近等基因系 AFLP技术 RT-PCR 生长发育过程 抗性基因 |
| 文章编号： | 0412-0914(2007)01-0109-04 |
| 收稿时间： | 2005-09-14 |
| 修稿时间： | 2005年9月14日 |
Differential genes expression of near-isogenic lines TcLr35 against wheat leaf rust |
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| CHEN Yun-fang,YANG Wen-xiang,YAN Hong-fei,LIU Li-qiang,CHU Dong,LIU Da-qun.Differential genes expression of near-isogenic lines TcLr35 against wheat leaf rust[J].Acta Phytopathologica Sinica,2007,37(1):109-112. |
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| Authors: | CHEN Yun-fang YANG Wen-xiang YAN Hong-fei LIU Li-qiang CHU Dong LIU Da-qun |
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| Institution: | Department of Plant Protection, Agricultural University of Hebei, Biological Control Center of Plant Diseases and Pests of Hebei Pronounce, Baoding 071001, China |
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| Abstract: | The differential genes expression of adult plant resistance gene of TcLr35 was analysed by using cDNA-AFLP technique.The results showed that 33 of 843 amplified bands were differential ones in different growth stages of TcLr35.A polymorphic band amplified by primer pair E-TG/M-CTG was found at different leaf-growing stages from the second to the sixth leaf of TcLr35 and absent in Thatcher and the seedling stage of TcLr35 on TcLr35 without inoculated with Puccinia recondita.Reverse Northern blotting test suggested that band E-TG/M-CTG-192 was a piece of special gene fragment of TcLr35.The band was cloned and sequenced subsequently.The fragment of 192 bp produced by E-TG/M-CTG contained an open reading frame(ORF),but did not show homologous to the published wheat gene sequences. |
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| Keywords: | genes expression cDNA-AFLP resistance gene TcLr35 |
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