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| 4个芦荟品种离体快繁技术研究 | |
| 引用本文： | 于春叶,马慧,张丽,田秀艳,钱浩洲,钟鸣,郭志富,陈丽静.4个芦荟品种离体快繁技术研究[J].辽宁林业科技,2009(4):13-16. |
| 作者姓名： | 于春叶 马慧 张丽 田秀艳 钱浩洲 钟鸣 郭志富 陈丽静 |
| 作者单位： | 1. 沈阳农业大学,辽宁省农业生物技术重点实验室,辽宁,沈阳,110161;山东省青岛市莱西七中,山东,青岛,266000 2. 沈阳农业大学,辽宁省农业生物技术重点实验室,辽宁,沈阳,110161 3. 沈阳市农业检测中心,辽宁,沈阳,110034 |
| 基金项目： | 辽宁省自然科学基金，辽宁省教育厅重点实验室项目 |
| 摘 要： | 该文对库拉索、中华、木立和高尚4个芦荟品种离体快繁技术进行了研究。结果表明，库拉索和木立芦荟不定芽间接分化，库拉索主要是丛生芽＋愈伤组织方式，木立芦荟主要是愈伤组织的形式；中华芦荟和高尚芦荟主要是丛生芽方式直接分化。4个芦荟品种的最适继代增殖培养基库拉索为MS＋6-BA2．0mg／L＋NAA0．1mg／L，繁殖系数为7．29；中华芦荟为MS＋6-BA1．0mg／L＋NAA1．5mg／L，繁殖系数达6．64；木立芦荟为MS＋6-BA2．0mg／L＋NAA0．1mg／L，繁殖系数达14．0；高尚芦荟为MS＋6-BA1．0mg／L＋NAA0．1mg／L，繁殖系数达8．10。库拉索和中华芦荟的最适生根培养基为MS＋NAA0．5mg／L，生根率分别为86．5％和100％；木立和高尚芦荟为MS，生根率分别为99％和79％。最适移栽基质为珍珠岩：河沙：草炭土=1：1：1，成活率达92％。 |
| 关 键 词： | 芦荟 分化途径 离体培养 快速繁殖 |
Studies on in vitro rapid propagation of 4 Aloe species |
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| YU Chun-ye,MA Hui,ZHANG Li,TIAN Xiu-yan,QIAN Hao-zhou,ZHONG Ming,GUO Zhi-fu,CHEN Li-jing.Studies on in vitro rapid propagation of 4 Aloe species[J].Liaoning Forestry Science and Technology,2009(4):13-16. | |
| Authors: | YU Chun-ye MA Hui ZHANG Li TIAN Xiu-yan QIAN Hao-zhou ZHONG Ming GUO Zhi-fu CHEN Li-jing |
| Institution: | 1.Liaoning Province Key Laboratory of Agriculture Biotechnology;Shenyang Agriculture University;Shenyang 110161;China;2.NO.7 Middle School of Laixi in Qingdao;Qingdao 266000;3.Shenyang Agricultural Examination Center;Shenyang 110034;China |
| Abstract: | In this paper,studies on in vitro propagation of 4 Aloe species were conducted.The results showed that: the main ways of dedifferentiation for Aloe vera,Aloe vera var.chinensis,Aloe arborescens and Aloe nobilis were adventitious shoots and callus,adventitious shoots,callus and adventitious shoots respectively.Regarding the optimal subculture media,Aloe barbadensis was Ms+6BA2.0+NAA0.1,the breeding coefficient was 7.29;Aloe vera var.chinensis was Ms+6BA1.0+NAA1.5,the breeding coefficient was 6.64;Aloe vera w... |
| Keywords: | Aloe ways of differentiation in vitro culture rapid propagation |
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