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羊和猪胎盘肽的提取
引用本文:周国华,孟凡军,王永志,葛利江.羊和猪胎盘肽的提取[J].动物医学进展,2005,26(9):88-89.
作者姓名:周国华  孟凡军  王永志  葛利江
作者单位:山东农业大学动物科技学院,山东,泰安,271018;山东省沂水县畜牧局,山东,沂水,276400;济南市农业学校,山东,济南,250023
摘    要:利用超滤法从羊和猪的胎盘中提取胎盘肽,用改良的加脲Tricine-SDS-PAGE电泳法对其进行初步定性鉴定。成功分离显示出了羊的胎盘肽,其分子质量小于5 ku,与人胎盘肽的分子质量范围完全符合。猪的6 ku的蛋白带很明显,但低于5 ku的蛋白带不是很明显。

关 键 词:羊胎盘肽  猪胎盘肽  加脲Tricine-SDS-PAGE电泳
文章编号:1007-5038(2005)09-0088-02
收稿时间:2005-04-25
修稿时间:2005年4月25日

Extration and Identification of Placental Peptide from Caprine and Swine
ZHOU Guo-hua,MENG Fan-jun,WANG Yong-zhi,GE Li-jiang.Extration and Identification of Placental Peptide from Caprine and Swine[J].Progress In Veterinary Medicine,2005,26(9):88-89.
Authors:ZHOU Guo-hua  MENG Fan-jun  WANG Yong-zhi  GE Li-jiang
Abstract:The distilled caprine and swine placental peptide was prepared by ultrafilter and identified by improved Tricine-SDS-PAGE eletrophoresis with urea in the experiment.Caprine placental peptide was prepared and separated,which had no more than 5 ku of molecular weight,according with the molecular weight range of human placental peptide completely.As to swine placental peptide,the electrophoretic lane of 6 ku molecular weight was obvious,but less than 5 ku was obscure.
Keywords:caprine placental peptide  swine placental peptide  tricine-SDS-PAGE with urea eletrophoresis
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