| 卷须链霉菌木聚糖酶B的纯化 |
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| 引用本文： | 丁长河,江正强,李里特,李秀婷,张艳.卷须链霉菌木聚糖酶B的纯化[J].中国农业大学学报,2003,8(6):1-4. |
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| 作者姓名： | 丁长河 江正强 李里特 李秀婷 张艳 |
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| 作者单位： | 中国农业大学,食品科学与营养工程学院,北京,100083 |
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| 基金项目： | 国家“十五”攻关课题 (2 0 0 1BA70 8B0 4 0 6 )，教育部研究重点项目 |
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| 摘 要： | 为了进一步研究链霉菌木聚糖酶的酶学性质，探讨了卷须链霉菌一种木聚糖酶的纯化方法。粗酶液经硫酸铵饱和度为20％～50％的分级沉淀和Q-sephrose FF离子交换柱层析2步纯化，得到一种电泳纯的木聚糖酶。定名为XynB；酶蛋白纯化倍数达到10．0倍，酶活力回收率达到24．0％。SDS-PAGE结果表明，该木聚糖酶的分子质量为22．0ku，属于低分子质量的木聚糖酶。
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| 关 键 词： | 卷须链霉菌 木聚糖酶B 纯化 硫酸铵沉淀 离子交换层析 |
| 文章编号： | 1007-4333(2003)06-0001-04 |
| 修稿时间： | 2003年5月10日 |
Purification of XynB from Streptomyces cirratus |
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| Ding Changhe,Jiang Zhengqiang,Li Lite,Li Xiuting,Zhang Yan.Purification of XynB from Streptomyces cirratus[J].Journal of China Agricultural University,2003,8(6):1-4. |
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| Authors: | Ding Changhe Jiang Zhengqiang Li Lite Li Xiuting Zhang Yan |
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| Abstract: | To study further the property of xylanases from Streptomyces, purification of a xylanase from Streptomyces cirratus D-10 was investigated. A xylanase, named XynB, was purified by stepwise ammonium sulphate precipitation (20%-50% saturation) and Q-sephrose FF ion exchange chromatography. After two-step treatment, ten-fold purification was obtained with 24.0% activity recovery. The purified XynB showed as a single protein band on SDS-PAGE that molecular weight of the enzyme was estimated to be 22.0 ku by SDS-PAGE, and belonged to one of lower molecular weight xylanases. |
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| Keywords: | Streptomyces cirratus xylanase purification ammonium sulphate precipitation ion exchange chromatography |
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