| 不同浓度激素对蕨菜愈伤组织诱导与增殖的影响 |
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| 引用本文： | 方利娟,苏仕林,蒲仕明. 不同浓度激素对蕨菜愈伤组织诱导与增殖的影响[J]. 湖北农业科学, 2012, 0(2): 406-408,425 |
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| 作者姓名： | 方利娟 苏仕林 蒲仕明 |
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| 作者单位： | 广西百色学院化学与生命科学系 |
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| 基金项目： | 广西教育厅科研项目(200807MS012) |
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| 摘 要： | 以蕨菜[Pteridium aquilinum(L.)kuhn]幼嫩根状茎为外植体,在愈伤组织诱导和增殖培养基中设置不同的6-BA和IBA浓度,研究6-BA和IBA对蕨菜愈伤组织诱导及增殖的影响。结果表明,1/2MS+0.2mg/L 6-BA+0.4 mg/L IBA为愈伤组织的最佳诱导培养基,诱导率为82%;1/2MS+0.2 mg/L IBA+0.2mg/L 6-BA为愈伤组织的最佳增殖培养基,增殖倍数为8.07。该研究结果为6-BA和IBA在蕨菜愈伤组织培养中的合理使用提供了参考。
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| 关 键 词： | 激素 蕨菜[Pteridium aquilinum(L.)kuhn] 愈伤组织 诱导 增殖 |
Effects of Hormones on Callus Induction and Propagation of Pteridium aquilinum(L.) kuhn |
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| FANG Li-juan,SU Shi-lin,PU Shi-ming. Effects of Hormones on Callus Induction and Propagation of Pteridium aquilinum(L.) kuhn[J]. Hubei Agricultural Sciences, 2012, 0(2): 406-408,425 |
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| Authors: | FANG Li-juan SU Shi-lin PU Shi-ming |
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| Affiliation: | (Department of Chemistry and Life Science,Baise University,Baise 533000,Guangxi,China) |
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| Abstract: | Using tender rhizomes of fern as explants,different concentration of 6-BA and IBA were added in callus induction and propagation mediums in order to study their effects on callus induction and propagation.The results indicated that 1/2 MS+0.2 mg/L 6-BA+0.4 mg/L IBA was the best callus induction medium with an induction rate of 82%;1/2 MS+0.2 mg/L IBA+0.2 mg/L 6-BA was the best callus proliferation medium as the callus was proliferated by 8.07 times.The results of this study could provide references for reasonable use of 6-BA and IBA in callus culture of Pteridium aquilinum. |
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| Keywords: | hormones Pteridium aquilinum(L.) kuhn callus induction propagation |
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