| 表达序列标签研究进展及其在甲壳动物中的应用概况 |
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| 引用本文： | 齐景斌,赵大显.表达序列标签研究进展及其在甲壳动物中的应用概况[J].湖北农业科学,2012(1):1-4. |
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| 作者姓名： | 齐景斌 赵大显 |
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| 作者单位： | 中国石油天然气管道工程有限公司;南昌大学食品科学与技术国家重点实验室;南昌大学生命科学与食品工程学院 |
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| 基金项目： | 南昌大学食品科学与技术国家重点实验室开放基金项目(SKLF-KF-201001) |
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| 摘 要： | 随着生物信息学的发展,表达序列标签(EST)在分子标记开发、新基因分离鉴定、基因表达谱分析、基因组功能注释、基因电子克隆等方面具有重要作用。简要介绍了EST分析的原理及其在基因识别、基因预测、物理图谱的构建、DNA芯片制备等方面的应用概况。综述了甲壳动物EST的研究现状,并对EST的应用前景进行了展望。
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| 关 键 词： | 表达序列标签(EST) 甲壳动物 生物信息学 |
Research Advance of Expressed Sequence Tag(EST) and Its Application in Crustacean |
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| QI Ji-bin,ZHAO Da-xian.Research Advance of Expressed Sequence Tag(EST) and Its Application in Crustacean[J].Hubei Agricultural Sciences,2012(1):1-4. |
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| Authors: | QI Ji-bin ZHAO Da-xian |
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| Institution: | 2a,2b(1.China Petroleum Pipeline Engineering Limited Corporation Langfang 065000,Hebei,China;2a.State Key Laboratory of Food Science and Technology;2b.College of Life Science and Food Engineering,Nanchang University,Nanchang 330031,China) |
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| Abstract: | With the development of bioinformatics,expressed sequence tag(EST) played an important role in molecular markers development,new genes isolation and identification,gene expression profile analysis,genome functional annotation and silico gene cloning.The principle of EST analysis and its applications in gene identification,gene prediction,physical map construction and DNA chip preparation was briefly introduced.In addition,the research status of crustacean EST and the prospect of EST application were also summarized. |
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| Keywords: | expressed sequence tag(EST) crustacean bioinformatics |
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