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| 分支杆菌DNA的不同提取方法在PCR反应中的定性评价 | |
| 引用本文： | 王海军,陈迪,王春雨,储结园,陈国栋,苏凤艳,王全凯.分支杆菌DNA的不同提取方法在PCR反应中的定性评价[J].经济动物学报,2010,14(2):107-112. |
| 作者姓名： | 王海军 陈迪 王春雨 储结园 陈国栋 苏凤艳 王全凯 |
| 作者单位： | 1. 吉林农业大学中药材学院,长春,130118 2. 内蒙古林西农牧业局,林西,025250 3. 吉林农业大学国际合作与交流处,长春,130118 |
| 基金项目： | 长春市科技局资助项目 |
| 摘 要： | 采用已报道过的分支杆菌DNA7种不同提取方法分别对标准BCG菌株和结核病鹿肺样本DNA提取后比较并进行聚合酶链反应(PCR)扩增,通过PCR反应对几种DNA提取方法做出定性评价。结果表明:方法5和7有100%临床样本检测率,方法7所得DNA浓度和纯度最高。说明临床样本分支杆菌DNA提取过程中使用物理和化学方法裂解细胞,使用蛋白酶K和氯仿纯化、异丙醇沉淀DNA是十分必要的。 |
| 关 键 词： | 分支杆菌 DNA提取 BCG 鹿肺样 PCR |
Qualitative Evaluation of Mycobacterial DNA Extraction Protocols for Polymerase Chain Reaction |
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| WANG Hai-jun,CHEN Di,WANG Chun-yu,CHU Jie-yuan,CHEN Guo-dong,SU Feng-yan,WANG Quan-kai.Qualitative Evaluation of Mycobacterial DNA Extraction Protocols for Polymerase Chain Reaction[J].Journal of Economic Animal,2010,14(2):107-112. | |
| Authors: | WANG Hai-jun CHEN Di WANG Chun-yu CHU Jie-yuan CHEN Guo-dong SU Feng-yan WANG Quan-kai |
| Institution: | WANG Hai-jun1,CHEN Di1,WANG Chun-yu1,CHU Jie-yuan1,CHEN Guo-dong2,SU Feng-yan1,WANG Quan-kai3(1.College of Chinese Medicinal Materials,Jilin Agricultural University,Changchun 130118,China,2.Agriculture and Animal Husbandry Bureau of Linxi County,Linxi 025250,3.International Cooperation and Communication Office,China) |
| Abstract: | This experiment compared the efficacy of various reported protocols for mycobacterial DNA extraction from standard BCG strains and lung samples of Deer tuberculosis and then qualitatively evaluates those extraction protocols by PCR assay.The results showed protocol 5 and 7 had 100% detection of clinical samples and protocol 7 had the highest concentration and purity than the other protocols.A physical and chemical treatment for lysis,use of proteinase K,DNA purification step with chloroform and DNA precipit... |
| Keywords: | BCG PCR |
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