Production of Middle White Piglets after Transfer of Embryos Produced In Vitro |
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Authors: | Koji MISUMI Yuri HIRAYAMA Misae SUZUKI Michiko NAKAI Hiroyuki KANEKO Junko NOGUCHI Kazuhiro KIKUCHI |
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Institution: | 1)Department of Technology, National Livestock Breeding Center, Fukushima 961-8511, Japan;2)Division of Animal Sciences, National Institute of Agrobiological Sciences, Ibaraki 305-8602, Japan;3)Present: National Institute of Agrobiological Sciences, Ibaraki 305-8602, Japan. |
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Abstract: | The present study was conducted to examine the feasibility of in vitro embryo production and transfer
technologies for producing Middle White piglets. After collection from three retired Middle White sows, a total of 222
oocytes were matured, fertilized and cultured in vitro, and a total of 50 embryos from the 4-cell to
blastocyst stage were produced by the 4th or 5th day. These embryos were transferred individually into three recipients along
with 5 in vivo-derived Duroc blastocysts. All of the recipients became pregnant, and they farrowed a total
of 9 Middle White and 9 Duroc piglets. These results suggest that in vitro embryo production using ovaries
from retired sows is useful for reproduction of pigs of pure breeds including the Middle White for breeding activities and
conservation/utilization of genetic resources. |
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Keywords: | Embryo transfer In vitro embryo production Middle White Piglet |
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