Delay in Cleavage of Porcine Embryos after Intracytoplasmic Sperm Injection (ICSI) Shows Poorer Embryonic
Development |
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| Authors: | Michiko NAKAI Manabu OZAWA Naoki MAEDOMARI Junko NOGUCHI Hiroyuki KANEKO Junya ITO Akira ONISHI Naomi KASHIWAZAKI Kazuhiro KIKUCHI |
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| Institution: | 1)Genetically Modified Organism Research Center, National Institute of Agrobiological Sciences, Ibaraki 305-0901, Japan;2)Division of Animal Sciences, National Institute of Agrobiological Sciences, Ibaraki 305-8602, Japan;3)Laboratory of Animal Reproduction, School of Veterinary Medicine, Azabu University, Kanagawa 252-5201, Japan |
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| Abstract: | In pigs, the embryonic developmental ability after intracytoplasmic sperm injection (ICSI) is inferior to that resulting from
in vitro fertilization (IVF). We evaluated the timing of cell division up to blastocyst formation on
embryonic development after ICSI using either whole sperm (w-ICSI) or the sperm head alone (h-ICSI) and IVF as a control. At
10 h after ICSI or IVF, we selected only zygotes, and each of the zygotes/embryos was evaluated for cleavage every 24 h until
168 h. We then observed a delay in the 1st and 2nd cleavages of h-ICSI embryos and also in blastocoele formation by w-ICSI
embryos in comparison with IVF embryos. The rate of blastocyst formation and the quality of blastocysts in both ICSI groups
were inferior to those in the IVF group. In conclusion, the delay in cleavage of porcine ICSI embryos shows poorer embryonic
development. |
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| Keywords: | Cleavage Embryonic development Intracytoplasmic sperm injection (ICSI) Pig |
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