FKBP46对飞蝗卵滞育具有促进作用

为验证FK506结合蛋白46(FK506 binding protein 46,FKBP46)在调节飞蝗Locusta migratoria滞育中的作用，首先从飞蝗中克隆FKBP46，然后合成双链RNA，将其注入到飞蝗雌成虫体内进行干扰，观察干扰后钙调磷酸酶（calcineurin,Ca N）的活性和卵滞育率的变化。结果显示，短日照条件下，RNA干扰FKBP46后飞蝗卵滞育率显著降低，由97.44%降至78.59%；在长日照条件下，RNA干扰FKBP46后，所有飞蝗卵均成功孵化，与对照组无显著差异；FKBP46基因沉默后，飞蝗后足中Ca N活性分别从0.24 U/g上调至0.29 U/g，脂肪体中Ca N活性从0.21 U/g上调至0.30 U/g。表明在短日照条件下FKBP46负调控Ca N影响飞蝗滞育，FKBP46的沉默效应表明该调控基因可能是基于RNA干扰的飞蝗滞育控制的理想靶点。

FKBP46 plays a role in promoting the egg diapause of the migratory locust, Locusta migratoria

The aim of the present study was to verify the function of FK506 binding protein 46 (FKBP46) in regulating the diapause of the migratory locust, Locusta migratoria. FKBP46 was cloned from L. migratoria and the double-stranded RNA of FKBP46 was synthesized, which was then injected into L. migratoria female adults to observe the changes in calcineurin (CaN) activity and the rate of egg diapause. The results showed that RNA interference of FKBP46 significantly decreased egg diapause rates from 97.44% to 78.59% under short photoperiods. Under long photoperiod, all L. migratoria eggs hatched after FKBP46 RNA interference, with no difference from the control group. Meanwhile, CaN activity was up-regulated from 0.24 U/g to 0.29 U/g in the hind leg and from 0.21 U/g to 0.30 U/g in the fat body, respectively, after FKBP46 gene silencing. These results suggested that FKBP46 negatively regulated CaN to affect L. migratoria diapause under short photoperiod. This regulatory gene is probably an ideal target for RNAi-based diapause control of the migratory locust.