| 转铜绿假单胞杆菌KatB基因烟草获得及其抗逆性鉴定 |
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| 引用本文： | 喻娜,程晨,孙辉,徐登献,祝建波. 转铜绿假单胞杆菌KatB基因烟草获得及其抗逆性鉴定[J]. 干旱区研究, 2011, 28(6): 1064-1070 |
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| 作者姓名： | 喻娜 程晨 孙辉 徐登献 祝建波 |
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| 作者单位： | 石河子大学生命科学学院 农业生物技术重点实验室，新疆 石河子 832003 |
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| 基金项目： | 国家转基因专项(2008ZX080052004); 兵团博士基金(2006jc07) |
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| 摘 要： | 以NC 89烟草（Nicotiana tabacum）为材料,通过农杆菌介导法获得转铜绿假单胞杆菌（Pseudomonas aerugi-nosa）KatB基因植株,利用PCR和RT-PCR对转化烟草进行鉴定,最后通过水分胁迫、盐胁迫、过氧化氢胁迫进行抗逆性分析。结果表明：与野生型植株相比,在缺水条件下的第13 d,3...
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| 关 键 词： | 铜绿假单胞杆菌 KatB基因 烟草 抗逆性 |
| 收稿时间： | 2010-11-20; |
Gain of the KatB Genetic Tobacco and Analysis on Stress Resistance of Transgenic Tobacco |
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| YU Na,CHENG Chen,SUN Hui,XU Deng-xian,ZHU Jian-bo. Gain of the KatB Genetic Tobacco and Analysis on Stress Resistance of Transgenic Tobacco[J]. Arid Zone Research, 2011, 28(6): 1064-1070 |
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| Authors: | YU Na CHENG Chen SUN Hui XU Deng-xian ZHU Jian-bo |
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| Affiliation: | Laboratory of Agricultural Biotechnology, College of Life Science, Shihezi University, Shihezi 832003, Xinjiang, China |
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| Abstract: | NC 89 tobacco plants were transformed via Agrobacterium tumefaciens.PCR and RT-PCR indicate that the foreign gene KatB of Pseudomonas aeruginosa has been integrated into the genome of tobacco.Stress resistance tests were carried out under water stress,300 mmol/L NaCl stress and 0.3 mmol/L H2O2 stress.The results proved that the physiological indexes including leaf relative water content,MDA activity,relative conductivity and maximum photochemical yield of PSII of KatB tobacco were evidently higher than thos... |
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| Keywords: | Pseudomonas aeruginosa KatB gene tobacco stress resistance |
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