| 利用cp DNA PCR-RFLP分析中国山楂属植物的亲缘关系 |
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| 引用本文： | 吴菲菲,张志宏,代红艳,张叶,常琳琳.利用cp DNA PCR-RFLP分析中国山楂属植物的亲缘关系[J].沈阳农业大学学报,2008,39(6). |
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| 作者姓名： | 吴菲菲 张志宏 代红艳 张叶 常琳琳 |
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| 作者单位： | 沈阳农业大学园艺学院,果树生物技术与遗传改良创新团队,沈阳,110161 |
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| 基金项目： | 教育部新世纪优秀人才支持计划项目
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| 摘 要： | 以8个山楂种的39份种质资源为试材,采用10对叶绿体DNA(chloroplast DNA,cp DNA)通用引物对总DNA进行扩增.并用7种限制性内切酶对PCR扩增产物酶切.结果表明:5对引物(cp01、cpP3、cp04、cp05、cp10)在山楂属植物上扩增出特异谱带,2种限制性内切酶(HinfⅠ和Taq Ⅰ)的酶切产物在供试材料间存在差异.山楂属种间存在较为丰富的cp DNA PCR-RFLP多态性,但山楂(C.pinnatifida)不同基因型间未检测到变异位点.麻核组(Sect.Santguineae)的甘肃山楂、光叶山楂、毛山楂、辽宁山楂和羽裂组(Sect.Pinnatifidae)的伏山植的cp DNA PCR-RFLP标记完全相同,但麻核组的阿尔泰山楂与其他山楂种的亲缘关系较远,距离系数达0.91.可见山楂属植物起源和演化的复杂性.伏山楂并非山楂的变种而很可能是一个新种.
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| 关 键 词： | 山楂属 亲缘关系 |
Genetic Relationships of Some Hawthorns (Crataegus spp.) Derived from cp DNA PCR-RFLP |
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| WU Fei-fei,ZHANG Zhi-hong,DAI Hong-yan,ZHANG Ye,CHANG Lin-lin.Genetic Relationships of Some Hawthorns (Crataegus spp.) Derived from cp DNA PCR-RFLP[J].Journal of Shenyang Agricultural University,2008,39(6). |
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| Authors: | WU Fei-fei ZHANG Zhi-hong DAI Hong-yan ZHANG Ye CHANG Lin-lin |
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| Institution: | WU Fei-fei,ZHANG Zhi-hong,DAI Hong-yan,ZHANG Ye,CHANG Lin-lin(College of Horticulture,Team of Biotechnology , Genetic Improvement of Fruit Trees,Shenyang Agricultural University,Shenyang 110161,China) |
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| Abstract: | Ten universal primer pairs of chloroplast genome were used to amplify cp DNA non-coding regions in eight Crataegus species including thirty-nine genotypes.The PCR products were digested by seven restriction enzymes.Five primer pairs(cp01,cp03,cp04,cp05 and cp10) generated special bands from Crataegus and the fragments digested by two restriction enzymes(Hinf I and TaqI) were different in Crataegus species.Interspecies of the genus Crataegus had higher cp DNA variations,but no visual bands were detected in d... |
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| Keywords: | cp DNA PCR-RFLP |
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