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中、草药水提取物抑菌活性的测定
引用本文:李永刚,文景芝.中、草药水提取物抑菌活性的测定[J].东北农业大学学报,2003,34(4):396-399.
作者姓名:李永刚  文景芝
作者单位:东北农业大学农学院植物保护系,黑龙江,哈尔滨,150030
基金项目:黑龙江省教育厅重大项目资助  95 5 / Z0 0 1,黑龙江省科技专项项目 GB0 1B3 0 1-0 7
摘    要:以辣椒疫霉菌、立枯丝核菌、番茄枯萎病菌、番茄早疫病菌、禾谷镰刀菌、大豆疫霉菌、番茄叶霉菌、烟草赤星病菌及玉米小斑病菌为供试菌种 ,用 30种中、草药的水提取物对其进行了抑菌活性测定。结果表明 ,大蒜提取液对立枯丝核菌、辣椒疫霉菌丝生长抑制率达 10 0 % ;猪胆汁对辣椒疫霉菌丝生长抑制率达 93.2 2 % ,对大豆疫霉菌丝生长抑制率达 80 .94 % ;金银花提取液对大豆疫霉菌丝生长抑制率达 10 0 % ;连翘提取液对大豆疫霉菌丝生长抑制率达 76 .10 % ;L S- 1为本课题组自行研制 ,由苦参、黄柏、蛇床子等 10余种中药组成 ,其对番茄早疫病菌、禾谷镰刀菌及大豆疫霉菌丝生长抑制率为 10 0 % ,对辣椒疫霉菌丝生长抑制率达 81.4 6 %以上 ,在病菌孢子萌发的抑制作用中对番茄枯萎病菌、番茄叶霉及玉米小斑病菌孢子萌发抑制率分别达到 90 .71% ,85 .71%和 77.14 %。

关 键 词:中、草药  抑菌作用  水提取物  测定
文章编号:1005-9369(2003)04-0396-04
修稿时间:2002年9月4日

Screening studies on fungistasis of water extracts of Chinese traditional medicine
LI Yong-gang,WEN Jing-zhi.Screening studies on fungistasis of water extracts of Chinese traditional medicine[J].Journal of Northeast Agricultural University,2003,34(4):396-399.
Authors:LI Yong-gang  WEN Jing-zhi
Institution:LI Yong-gang,WEN Jing-zhi *
Abstract:The fungistasis of 30 water extracts of Chinese traditional medicine were tested with Rhizoctonia solani,Fusarium graminicola,Phytophthora capsici,Fusarium oxysporum,Phytophthora sojae,Alternaria solani,Fulria fulva,Alternaria alternata,Bipolaris maydis.Results showed that Bulbus Allii extracts had 100% inhibiting rates to the hypha growth of Rhizoctonia solani,Phytophthora capsic;Choler had 93.22% inhibiting rates to the hypha growth of Phytophthora capsic,80.94% to Phytophthora sojae;Honeysuckle extracts had 100% inhibiting rates to the hypha growth of Phytophthora sojae;Fructus Forsythiae extracts had 76.10% inhibiting rates to the hypha growth of Phytophthora sojae.LS-1 was a kind of fungicide which was compound of several plant extracts by our task group.LS-1 had 100% inhibiting rates to the hypha growth of Alternaria solani,Fusarium graminicola,Phytophthora sojae,had 81.46% to Phytophthora capsic.LS-1 had 90.71%,85.71% and 74.42% inhibiting rates to the sprouting of Fusarium oxysporum,Fulria fulva,Bipolaris maydis.
Keywords:Chinese traditional medicine  fungustasis bioactivity-screening  water extracts  mensuration
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