Genetic diversity in landrace populations of Hordeum vulgare L. from Sardinia, Italy, as revealed by RAPDs, isozymes and morphophenological traits

Barley is the second most widely cultivated cereal on the island of Sardinia. It is grown for green fodder, grain and straw, all of which are used for animal feet. The cultivation of modem varieties is limited and many farmers grow local populations of the six-row landrace ‘S'orgiu sardu’. Twelve local populations (20 strains per population) were evaluated for 13 random amplified polymorphic DNA (RAPD) markers, sisisozyme loci and five morphophenological traits. Results showed that Sardinian barley is characterized by a lax six-row spike and is. in general, a spring type. Isozyme polymorphism was delected for only two esterase loci (Est and Est 4). The genetic variation for all types of murkers, was found located mostly within population (about 84%, for isozymes aud morphaphenological traits and 89%, for RAPDs). This finding appears to confirm the hypothesis that the populations studied belong to the same land race called ‘S'orgiu sardu’ by farmers. A geographical differential ion between southern and northern populations was foand in relation to distinct environmental characteristics. Taking into account all types of marker 209 (90.5%) different genotypes were identified out of 231 individuals. The overall results confirm the high variability that can be found within landrace populations, underlining the value of landraccs as a source of genetic variation.