The effects analysis of two neonicotinoid insecticides on in
vitro maturation of porcine oocytes using hanging drop monoculture
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Authors: | Sadamasa ISHIKAWA Kou HIRAGA Yuuki HIRADATE Kentaro TANEMURA |
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Affiliation: | 1)Laboratory of Animal Reproduction and Development, Graduate School of Agricultural Science, Tohoku University, Sendai 981–8555, Japan |
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Abstract: | Acetamiprid (ACE) and imidacroprid (IMI) are known neonicotinoid insecticides with strongaffinities for the insect-selective nicotinic acetylcholine receptor. These provide insectcontrol by hyperstimulating insect nerves and are used for agricultural pest management.However, it has also been reported that ACE and IMI affect mammalian reproductivefunction. We determined the effects of ACE and IMI on the in vitromaturation of porcine oocytes. Significant decreases in nuclear maturation rates wereobserved in the ACE or IMI-exposed groups. Also, in matured oocytes from the ACE orIMI-exposed groups, irregular chromosomes were observed. Our results suggest that ACE andIMI exposure was detrimental to porcine oocytes and the extent of the effects depends onthe concentration of exposure. |
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Keywords: | hanging drop in vitro maturation (IVM) neonicotinoid porcine oocyte |
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