Isolation and characterization of arylacetamide deacetylase in cynomolgus
macaques |
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| Authors: | Yasuhiro UNO Masakiyo HOSOKAWA Teruko IMAI |
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| Institution: | 1)Pharmacokinetics and Bioanalysis Center, Shin Nippon Biomedical Laboratories, Ltd., Wakayama 642–0017, Japan;2)Laboratory of Drug Metabolism and Biopharmaceutics, Faculty of Pharmaceutical Sciences, Chiba Institute of Science, Choshi, Chiba 288–0025, Japan;3)Graduate School of Pharmaceutical Sciences, Kumamoto University, Kumamoto 862–0973, Japan |
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| Abstract: | Arylacetamide deacetylase (AADAC), a microsomal serine esterase, hydrolyzes drugs, such
as flutamide, phenacetin and rifampicin. Because AADAC has not been fully investigated at
molecular levels in cynomolgus macaques, the non-human primate species widely used in drug
metabolism studies, cynomolgus AADAC cDNA was isolated and characterized. The deduced
amino acid sequence, highly homologous (92%) to human AADAC, was more closely clustered
with human AADAC than the dog, rat or mouse ortholog in a phylogenetic tree.
AADAC was flanked by AADACL2 and
SUCNR1 in the cynomolgus and human genomes. Moreover, relatively
abundant expression of AADAC mRNA was found in liver and jejunum, the drug-metabolizing
organs, in cynomolgus macaques, similar to humans. The results suggest molecular
similarities of AADAC between cynomolgus macaques and humans. |
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| Keywords: | arylacetamide deacetylase cynomolgus monkey drug metabolism genome tissue expression |
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