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| 空间诱变水稻多蘖矮突变体不同分蘖期蛋白质组研究 | |
| 引用本文： | 王巍,魏力军,王俊敏,徐建龙,孙野青. 空间诱变水稻多蘖矮突变体不同分蘖期蛋白质组研究[J]. 核农学报, 2011, 25(3): 405-415 |
| 作者姓名： | 王巍 魏力军 王俊敏 徐建龙 孙野青 |
| 作者单位： | 1. 哈尔滨工业大学生命科学与工程系,黑龙江哈尔滨,150001 2. 浙江省农业科学院作物与核技术应用研究所,浙江杭州,310021 3. 中国农业科学院作物科学研究所,北京,100081 4. 哈尔滨工业大学生命科学与工程系,黑龙江哈尔滨150001;大连海事大学环境系统生物学研究所,辽宁大连116026 |
| 基金项目： | 国家863项目(2008AA7035020E); 国家科技支撑计划重点项目(2008BAD97B02) |
| 摘 要： | JB-1返回式卫星搭载水稻(Oryza sativa L.)丙95-503干种子,地面种植后筛选出多蘖矮杆突变体R955.利用荧光差异显示双向电泳(2-D DIGE)对突变体播种后第14天(未发生分蘖)、第21天(分蘖起始)、第55天(最高分蘖期)3个营养生长期叶片总蛋白进行分离及定量分析(未搭载植株为对照),检测到在... |
| 关 键 词： | 水稻 多蘖矮 空间诱变 蛋白质组 S-类核糖核酸酶 |
| 收稿时间： | 2010-09-17 |
| 修稿时间： | 2010-12-08 |
COMPARATIVE PROTEOMIC ANALYSIS OF A HIGH-TILLERING DWARF MUTANT INDUCED BY SPACEFLIGHT AT DIFFERENT TILLERING STAGES |
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| WANG Wei,WEI Li-jun,WANG Jun-min,XU Jian-long,SUN Ye-qing. COMPARATIVE PROTEOMIC ANALYSIS OF A HIGH-TILLERING DWARF MUTANT INDUCED BY SPACEFLIGHT AT DIFFERENT TILLERING STAGES[J]. Acta Agriculturae Nucleatae Sinica, 2011, 25(3): 405-415 | |
| Authors: | WANG Wei WEI Li-jun WANG Jun-min XU Jian-long SUN Ye-qing |
| Affiliation: | WANG Wei1 WEI Li-jun1 WANG Jun-min2 XU Jian-long3 SUN Ye-qing1,4(1.Department of Life Science and Engineering,Harbin Institute of Technology,Harbin,Heilongjiang 150001,2.Institute of Crop and Nuclear Technology Utilization,Zhejiang Academy of Agricultural Sciences,Hangzhou,Zhejiang 310021,3.Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,4.Institute of Environmental Systems Biology,Dalian Maritime University,Dalian,Liaoning 116026) |
| Abstract: | To investigate changes of proteins during rice tiller development,a comparative proteomic analysis between a high-tillering dwarf mutant R955 induced by spaceflight and its ground control was performed at three developmental stages during the vegetative growth,i.e.at days 14(no tiller appearance),21(initial tillering) and 55(maximum tillering) after sowing.Analysis of the protein spots on two-dimensional fluorescence difference gel electrophoresis 2-D DIGE images revealed 97 proteins that were differentiall... |
| Keywords: | rice tillering dwarf space mutation proteome S-like RNase |
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