| Separation and purification of Arabinogalactan obtained from Larix gmelinii by macroporous resin adsorption |
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| 作者单位： | HUANG Zhan-hua，FANG Gui-zhen，ZHANG Bin Northeast Forestry University，Key Laboratory of Bio-based Material Science and Technology，Ministry of Education，Harbin 150040，P. R. China |
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| 基金项目： | Important Tackle Key Problem of Heilongjiang Province，东北大学校科研和教改项目 |
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| 摘 要： | Arabinogalactan （AG） obtained from Larix gmelinii R. waS purified with the method of macroporous resin adsorption. Effects of various parameters on the adsorption, including adsorption time and temperature, the concentration and the dosage of raw AG the reused numbers of resin, were investigated. The effect of purification was tested through the removal rate of impurity and the contents of AG and impurity. The optimal condition was determined as follows： adsorbed at 30℃ for 2 h with the concentration of raw AG 〈0.1 g·mL^-1 and its dosage 〈 7 mL, the dose of resin was 3 g and reused for 4 times. On the basis of these, macroporous resin column was used for AG purification. The result showed that the AG yield could reach 68.28% with sugar content of 95.02%. The analysis of IR and UV showed that the effect of macroporous resin characteristics on the purification of AG was significant. The obtained product had the same functional groups with standard sample.
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| 关 键 词： | 落叶松 阿拉伯半乳聚糖 大孔树脂吸附法 分离 纯化 |
| 收稿时间： | 2006-09-26 |
| 修稿时间： | 2006-10-30 |
Separation and purification of Arabinogalactan obtained from Larix gmelinii by macroporous resin adsorption |
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| Huang Zhan-hua,Fang Gui-zhen,Zhang Bin. Separation and purification of Arabinogalactan obtained from Larix gmelinii by macroporous resin adsorption[J]. Journal of Forestry Research, 2007, 18(1): 81-83. DOI: 10.1007/s11676-007-0016-x |
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| Authors: | Huang Zhan-hua Fang Gui-zhen Zhang Bin |
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| Affiliation: | (1) Northeast Forestry University, Key Laboratory of Bio-based Material Science and Technology, Ministry of Education, Harbin, 150040, P. R. China |
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| Abstract: | Arabinogalactan (AG) obtained from Larix gmelinii R. was purified with the method of macroporous resin adsorption. Effects of various parameters on the adsorption, including adsorption time and temperature, the concentration and the dosage of raw AG, the reused numbers of resin, were investigated. The effect of purification was tested through the removal rate of impurity and the contents of AG and impurity. The optimal condition was determined as follows: adsorbed at 30℃ for 2 h with the concentration of raw AG ＜0.1 g·mL-1 and its dosage ＜ 7 mL, the dose of resin was 3 g and reused for 4 times. On the basis of these, macroporous resin column was used for AG purification. The result showed that the AG yield could reach 68.28% with sugar content of 95.02%. The analysis of IR and UV showed that the effect of macroporous resin characteristics on the purification of AG was significant. The obtained product had the same functional groups with standard sample. |
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| Keywords: | Arabinogalactan Larix gmelinii R. Macroporous resin |
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