| 北美柔枝松组织培养研究 |
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| 引用本文： | 李玲莉,郭素娟,李吉跃.北美柔枝松组织培养研究[J].北京林业大学学报,2010,32(3):209-212. |
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| 作者姓名： | 李玲莉 郭素娟 李吉跃 |
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| 作者单位： | 1 北京林业大学省部共建森林培育与保护教育部重点实验室 2 华南农业大学林学院 |
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| 基金项目： | "948"国家林业局引进项目 |
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| 摘 要： | 以柔枝松的幼嫩顶芽为外植体,对不定芽的诱导、增殖和生根条件进行筛选。结果发现:在附加0.5mg/LBA和0.1mg/LNAA的SH培养基中,不定芽的诱导率为86.67%;诱导4周后,将外植体继代于SH基本培养基中,使不定芽伸长生长;然后,将伸长的不定芽转至含有2.0mg/LBA的SH培养基中进行增殖培养,4周后,增殖系数为3.75;继而在SH基本培养基或含有0.05%活性炭的SH培养基中进行壮苗培养;当不定芽长至1～1.5cm时,转入无植物生长调节剂的1/2GD培养基中进行生根诱导,培养6周后,在不定芽基部形成1～3条不定根;炼苗后,植株在土壤中(V(草炭土)∶V(蛭石)=1∶1)移栽成活率为64.86%。
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| 关 键 词： | 柔枝松 不定芽诱导 不定芽增殖 生根 |
| 收稿时间： | 1900-01-01 |
Tissue culture protocol of limber pine of North America |
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| LI Ling-li,GUO Su-juan,LI Ji-yue.Tissue culture protocol of limber pine of North America[J].Journal of Beijing Forestry University,2010,32(3):209-212. |
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| Authors: | LI Ling-li GUO Su-juan LI Ji-yue |
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| Institution: | 1 Key Laboratory for Silviculture and Conservation of Ministry of Education, Beijing Forestry University, 100083，P. R. China； 2 College of Forestry, South China Agricultural University, Guangzhou, 510642, P. R. China. |
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| Abstract: | The tender apical bud of limber pine was taken as explant to investigate the induction and multiplication of adventitious buds and the formation of adventitious roots.It was found that the induction frequency of adventitious buds was 86.67% when the tender apical bud was cultured on SH medium supplemented with 0.5 mg/L BA and 0.1 mg/L NAA.Four weeks later,the induced buds were subcultured on SH basal medium for elongation.Then the elongated shoots were transferred to SH medium containing 2.0 mg /L BA for mu... |
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| Keywords: | limber pine(Pinus flexilis James) induction of adventitious buds multiplication of adventitious buds rooting |
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