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远海梭子蟹成熟卵巢和早期胚胎的脂类成分比较研究
引用本文:周波,吴旭干,丰浪,成永旭,杨筱珍,刘智俊. 远海梭子蟹成熟卵巢和早期胚胎的脂类成分比较研究[J]. 上海水产大学学报, 2011, 0(6)
作者姓名:周波  吴旭干  丰浪  成永旭  杨筱珍  刘智俊
作者单位:上海海洋大学省部共建水产种质资源发掘与利用教育部重点实验室;
基金项目:上海市晨光计划(2007CG64); 国家教育委员会博士点基金(200802640002); 上海市高校创新团队项目(D-8006-10-209)
摘    要:测定和比较了远海梭子蟹(Portunus pelagicus)成熟卵巢和早期胚胎的水分、总脂、脂类组成和脂肪酸含量,结果表明:早期胚胎的水分含量显著大于成熟卵巢,但后者总脂/组织湿重含量显著小于前者,两者的总脂/组织干重并无显著差异;甘油三酰和磷脂含量在早期胚胎与成熟卵巢之间并不存在显著差异,但是早期胚胎中的游离脂肪酸和胆固醇含量均显著高于成熟卵巢,这可能是胚胎发育过程中脂类物质被水解成游离脂肪酸、胆固醇和甘油等物质;就脂肪酸组成而言,两者中均检测出27种已知脂肪酸,仅15∶0、14∶1n7、20∶1n7和18∶3n3 4种脂肪酸的含量存在显著差异(P<0.05),16∶0、18:0、16∶1n7、18∶1n9、20∶5n3和22∶6n3为成熟卵巢和早期胚胎中的主要的脂肪酸种类,其中n3-PUFA/n6-PUFA和20∶5n3/22∶6n3分别为3.5和0.7。

关 键 词:远海梭子蟹  成熟卵巢  早期胚胎  脂肪酸  脂类组成  

A comparative study of lipid class and fatty acid composition of mature ovary and early embryo of Portunus pelagicus
ZHOU Bo,WU Xu-gan,FENG Lang,CHENG Yong-xu,YANG Xiao-zhen,LIU Zhi-jun. A comparative study of lipid class and fatty acid composition of mature ovary and early embryo of Portunus pelagicus[J]. Journal of Shanghai Fisheries University, 2011, 0(6)
Authors:ZHOU Bo  WU Xu-gan  FENG Lang  CHENG Yong-xu  YANG Xiao-zhen  LIU Zhi-jun
Affiliation:ZHOU Bo,WU Xu-gan,FENG Lang,CHENG Yong-xu,YANG Xiao-zhen,LIU Zhi-jun(The Key Laboratory of Exploration and Utilization of Aquatic Genetic Rescours,Shanghai Ocean University,Ministry of Education,Shanghai 201306,China)
Abstract:The present experiments were conducted to investigate the moisture content,lipid class and fatty acid composition in mature ovary and early embryo of Portunus pelagicus.The results indicated that:(1) Although the early embryo of P.pelagicus had higher moisture content than that of mature ovary,the higher total lipid/wet tissue could be found in the mature ovary than that of early embryo;(2) There were no significatn differences in the triacyglycerol(TG) and phospholipids(PL) contents between mature ovary an...
Keywords:Portunus pelagicus  mature ovary  early embryo  fatty acid  lipid class composition  
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