| 球孢白僵菌不同世代菌株胞外蛋白酶与毒力的关系 |
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| 引用本文： | 彭国雄,张永军,杨星勇,王中康,裴炎.球孢白僵菌不同世代菌株胞外蛋白酶与毒力的关系[J].中国生物防治,2000,16(2):61-64. |
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| 作者姓名： | 彭国雄 张永军 杨星勇 王中康 裴炎 |
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| 作者单位： | 西南农业大学生物技术中心,重庆北碚,400716 |
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| 基金项目： | 国家自然科学基金，重庆市应用基础研究资助项目 |
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| 摘 要： | 以蝉蜕、蝇明壳两种培养基培养球孢白僵菌不同世代菌株分析其类枯草杆菌蛋白（Ｐｒ１）和总胞外蛋白酶的产生水平及其与菌株毒力的相关性。结果表明，菌株的Ｐｒ１产生水平达到最高的时间较总胞外蛋白酶早１～２天，在单蜕培养基中，菌株Ｐｒ１产生水平较高，而总胞外蛋白产生水平大大低于蝇明壳培养基中的产生水平。随民代数的增加，Ｐｒ１和总胞外蛋白酶产生水平与其毒力的相关系数分别为０．９２和０．８９，高于 包外蛋白酶与其
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| 关 键 词： | 球孢白僵菌 类枯草杆菌蛋白酶 胞外蛋白酶 毒力 |
Relationship of the Production of Subtilisin-like Protease and Total Extracellular Protease by Beauveria bassiana with Its Virulence to Bombyx mori |
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| PENG Guoxiong,ZHANG Yongjun,YANG Xingyong,WANG Zhongkang,PEI Yan.Relationship of the Production of Subtilisin-like Protease and Total Extracellular Protease by Beauveria bassiana with Its Virulence to Bombyx mori[J].Chinese Journal of Biological Control,2000,16(2):61-64. |
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| Authors: | PENG Guoxiong ZHANG Yongjun YANG Xingyong WANG Zhongkang PEI Yan |
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| Abstract: | The production of subtilisin like protease (Prl) and total extracellular protease from different subcultures of Beauveria bassiana was investigated in cicada exuviae and maggot cuticle liquid culture. The relationship between protease production and virulence to Bombyx mori was compared. The results showed that Prl produced earlier than that of total extracellular protease, specially in cicada exuviae culture. The proteases production by different subcultures was significantly related to their virulence toB.mori. The relationship between Prl production with virulence was higher than that of total extracellular protease. |
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| Keywords: | Beauveria bassiana subtilisin like protease total extracellular protease virulence |
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