紫穗槐多倍体诱导研究 |
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引用本文: | 王欢,张旋,张金凤. 紫穗槐多倍体诱导研究[J]. 安徽农业科学, 2010, 38(21): 11067-11068,11089. DOI: 10.3969/j.issn.0517-6611.2010.21.013 |
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作者姓名: | 王欢 张旋 张金凤 |
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作者单位: | 北京林业大学林木、花卉遗传育种教育部重点实验室,北京,100083;北京林业大学林木、花卉遗传育种教育部重点实验室,北京,100083;北京林业大学林木、花卉遗传育种教育部重点实验室,北京,100083 |
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摘 要: | [目的]研究紫穗槐(Amorpha fruticosa L.)多倍体诱导的最优条件。[方法]选取秋水仙素浓度、光照时间和根尖长度3个因素,每个因素3个水平,采用L9(34)正交试验设计,对紫穗槐多倍体诱导条件进行优化。[结果]紫穗槐根尖诱导的最佳条件为:秋水仙素的浓度为0.050%,无光照,根尖长度为0~4mm。[结论]该研究可为开展紫穗槐多倍体育种奠定基础。
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关 键 词: | 紫穗槐 秋水仙素 多倍体 |
Study on Induction of Polyploidy in Amorpha fruticosa L. |
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Affiliation: | WANG Huan et al(Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants,Ministry of Education,Beijing Forestry University,Beijing 100083) |
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Abstract: | [Objective] The research aimed to study the optimal conditions of polyploidy induction in Amorpha fruticosa L..[Method] Three levels factors of each factors,colchicine concentration,illumination time and temperature,were chose to carry out L9(34)orthogonal design to optimize the polyploidy induction conditions in Amorpha fruticosa L..[Result] The results showed that optimal conditions of polyploidy induction in Amorpha fruticosa L.was as follow:the colchicine concentration 0.050%,darkness and the root tip length 0-4 mm.[Conclusion] The study can lay basis for polyploidy breeding of Amorpha fruticosa L.in future. |
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Keywords: | Amorpha fruticosa L. Colchicines Polyploidy |
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