Myosin V walks hand-over-hand: single fluorophore imaging with 1.5-nm localization |
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Authors: | Yildiz Ahmet Forkey Joseph N McKinney Sean A Ha Taekjip Goldman Yale E Selvin Paul R |
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Institution: | Center for Biophysics and Computational Biology, University of Illinois, Urbana-Champaign, IL 61801, USA. |
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Abstract: | Myosin V is a dimeric molecular motor that moves processively on actin, with the center of mass moving approximately 37 nanometers for each adenosine triphosphate hydrolyzed. We have labeled myosin V with a single fluorophore at different positions in the light-chain domain and measured the step size with a standard deviation of <1.5 nanometers, with 0.5-second temporal resolution, and observation times of minutes. The step size alternates between 37 + 2x nm and 37 - 2x, where x is the distance along the direction of motion between the dye and the midpoint between the two heads. These results strongly support a hand-over-hand model of motility, not an inchworm model. |
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