丛枝菌根真菌和有机土基质栽培对甜椒根际微生物分子多态性的影响(英文)

通过采用变性梯度凝胶电泳(DGGE)技术对普通土、普通土+G.M、有机土和有机土+G.M 四个不同栽培处理下甜椒根际土壤微生物的多样性进行研究,结果表明:接菌处理使细菌的种类在数量上有所增加,其中有新的细菌类型的出现,也有一些细菌类型的减少和消失,一些共有细菌种类的数量也得到了丰富;应用有机土栽培有利于一些菌群的生长繁殖,有助于促成优势菌群的建立;对电泳图谱的相似性系数分析表明,有机土对细菌菌群多样性的影响强于接种菌根真菌处理;普通土接种菌根真菌对土壤微生物种群的影响强于有机土;接种菌根真菌使土壤的细菌种群结构发生了变化,并增加其菌群种类的相似性。

Effects of Arbuscular Mycorrhizal Fungus and Organic Substrate on the Molecular Diversity of Microbes in Sweet Pepper Rhizosphere under Protected Cultivation

Denaturing gradient gel electrophoresis(DGGE) was used to study the effects of different soil additives,i.e.control soil + Glomus mossea-2(G.M),organic substrate,organic substrate + G.M,on microbial molecular diversity in sweet pepper rhizosphere under protected cultivation.Results showed that following the inoculation of G.M,the total number of bacterial species increased,with the appearance and disappearance of some bacterial species.Some bacteria,though detected in all treatments,showed increased cell/clone numbers with the addition of arbuscular mycorrhizal fungus G.M.The organic substrate favored the growth and propagation of some bacteria and was advantageous to the establishment of some dominant microbial flora.Resulted from the similarity analyses of the DGGE profile,it was obvious that organic substrate had more influence on the diversity of rhizosphere microbial flora than the inoculation of arbuscular mycorrhizal fungus G.M.Meanwhile,bacterial diversity in the control soil inoculated with G.M was higher than the organic soil.It was thus concluded that the inoculation of G.M changed the structure of microbial population in sweet pepper rhizosphere and increased the similarity of the rhizosphere bacterial species.