奶牛亚洲Ⅰ型口蹄疫免疫程序的研究

用液相阻断ELISA(酶联免疫吸附试验)方法对北京市怀柔地区重点饲养奶牛场户进行亚洲I型口蹄疫(FMD)母源抗体、免前和免后抗体水平进行调查,以探寻牛体内FMD抗体消长规律,在此基础上制定科学的免疫程序,从而指导怀柔地区奶牛FMD的免疫。结果发现母源抗体水平在0日龄最高,以后逐步下降,高水平抗体一直维持到56日龄,86日龄时回落至1∶93(<1∶128),90日龄首免后,抗体滴度开始回升,28d后加强免疫,抗体滴度大幅度升高,抗体效价离度降低。说明FMD首免的适宜日龄为90日龄,适时地加强免疫有利于牛群抵抗FMD;FMD疫苗对育成、青年和成年期的奶牛都有理想的免疫效果。

Study on Cows Asia I Foot-and-mouth Disease Immunization Program

With liquid block ELISA test method investigating the foot-and-mouth disease ( FMD ) antibodies of focus cow breeding farms and farmers, the source from the front and the antibodies against the level in Huairou area of Beijing, to devise the body, and the antibodies FMD succeeding. On the basis of immunity program for scientific and guidance mollification area of cows FMD immunization. The results found that the source in the highest level of antibody 0 age, later, antibodies have been maintaining at high levels of age when 86, 56 age to a less than 1 ∶ 93 (<1∶128 ), 90 days after the first from age and the antibodies drops began to increase, 28 d after the big drop of immunity, antibodies, antibodies results from the lower price. Keywrods cow; Asia I; foot-and-mouth disease; antibodies monitoring; immunization program