Ca~(2+)对水稻Ac/Ds愈伤组织增殖·分化及生根的影响

目的]研究Ca2+对水稻Ac/Ds愈伤组织增殖、分化及生根的影响。方法]选用水稻品种Dongjin的Ac/Ds插入突变株系的种子,从成熟胚盾片诱导出愈伤组织,在筛选出的最适增殖、分化及生根培养基中添加不同浓度的Ca2+,研究Ca2+对水稻愈伤组织增殖、分化及生根的影响。结果]适当增加Ca2+浓度能提高水稻愈伤组织的相对生长量、分化率、再生植株生根条数及根长,当Ca2+浓度为6.0mmol/L时效果最佳,当Ca2+浓度为7.5~15.0 mmol/L时水稻愈伤组织的相对生长量、分化率、再生植株的生根条数及根长呈下降趋势。结论]适当增加培养基中Ca2+浓度可提高水稻Ac/Ds愈伤组织的相对生长量、分化率、再生植株生根条数及根长。

Effect of Ca2+ Proliferation, Differentiation and Rotting of Callus of Rice with Ac/Ds Elements

Objective] The study was to research the effect of Ca2 on proliferation,differentiation and rooting of callus of rice mutants with Ac/Ds.Method] The seeds of rice mutants with Ac/Ds elements inserted from rice variety Dongjin were selected and the callus were induced from scutellum of mature embryo.Ca2 with different concn.was added into the selected optimum enrichment,differentiation and rooting medium to study the effect of Ca2 on proliferation,differentiation and rooting of rice callus.Result] The relative growth rate and differentiation rate of rice callus and the rooting number and root length of regenerated plant could be increased when Ca2 concn.was increased properly,and the effect was better when Ca2 was 6.0 mmol/L,but the relative growth rate and differentiation rate of rice callus and the rooting number and root length of regenerated plant showed downward trend when Ca2 was 7.5-15.0 mmol/L.Conclusion] The relative growth rate and differentiation rate of callus of rice mutants with Ac/Ds elements and the rooting number and root length of regenerated plant could be increased when Ca2 concn.was increased properly.