| 利用RAPD标记鉴定和区分梅花42个宫粉型品种 |
| |
| 引用本文： | 张俊卫,柴玉荣,包满珠. 利用RAPD标记鉴定和区分梅花42个宫粉型品种[J]. 园艺学报, 2004, 31(4): 487-490 |
| |
| 作者姓名： | 张俊卫 柴玉荣 包满珠 |
| |
| 作者单位： | (华中农业大学园艺林学学院，园艺植物生物学教育部重点实验室，武汉430070) |
| |
| 基金项目： | 国家自然科学基金(30170666)项目，教育部骨干教师资助计划项目，高校博士点基金资助项目 |
| |
| 摘 要： | 利用RAPD标记，对形态特征相似的42个宫粉型梅花品种的基因组DNA进行随机引物PCR扩增。从120个引物中筛选出21个引物，共扩增出96条谱带，其中多态性谱带68条，占扩增谱带数的70．8％ ，品种问相似系数范围为0．737～0．961。在21个引物中，7个引物扩增的谱带可区分27个品种，7个引物相互组合可区分其余的l5个品种。梅花品种RAPD指纹图谱的建立可以为品种知识产权保护提供依据。
|
| 关 键 词： | 梅花 宫粉型 RAPD 指纹图谱 |
| 文章编号： | 0513-353X(2004)04-0487-04 |
RAPD Identification and Discrimination of 42 Ornamental Pink Double Form Cultivars of Prunus mume Sieb.et Zucc. |
| |
| Zhang Junwei,Chai Yurong,and Bao Manzhu. RAPD Identification and Discrimination of 42 Ornamental Pink Double Form Cultivars of Prunus mume Sieb.et Zucc.[J]. Acta Horticulturae Sinica, 2004, 31(4): 487-490 |
| |
| Authors: | Zhang Junwei Chai Yurong and Bao Manzhu |
| |
| Affiliation: | (Key Laboratory of Horticultural Plant Biology，Ministry of Education，College of Horticulture and Forestr) Sciences，HuazhongAgricultural University，Wuhan 430070，China) |
| |
| Abstract: | The genomic DNA was used as template and random primers were used to develop fingerprinting of 42 cultivars of Pink Double Form of Prunus mume Sieb. et Zucc. by PCR. Of the 120 primers screened, 21 primers can generate 96 fragments, 70. 8% of which were polymorphic ones. The similarity coefficient of cultivars was between 0. 737 -0. 961. The 42 cultivars can be identified and discriminated efficiently with seven primers. The establishment of RAPD fingerprinting is applicable in cultivar identification and breeder's rights protection of Prunus mume. |
| |
| Keywords: | Prunus mume Pink Double Form RAPD Fingerprinting |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《园艺学报》浏览原始摘要信息 |
|
点击此处可从《园艺学报》下载全文 |
