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Manufacturing polymethyl methacrylate nanofibers as a support for enzyme immobilization |
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| Authors: | Navid Amini Saeedeh Mazinani Seyed-Omid Ranaei-Siadat Mohammadreza Kalaee Kaveh Niknam Vida Adlfar |
| Institution: | 4. Department of Polymer Engineering, South Tehran Branch, Islamic Azad University, 1777613651, Tehran, Iran 1. Amirkabir Nanotechnology Research Institute (ANTRI), Amirkabir University of Technology, 15875-4413, Tehran, Iran 2. NanoBiotechnology Engineering Lab., Department of Biotechnology, Faculty of Energy Engineering and New Technologies, Shahid Beheshti University, GC, Tehran, Iran 3. Polymer Engineering Group, Department of Engineering, Qom University of Technology, Qom, Iran |
| Abstract: | Nanofibers have a great potential for enzyme immobilization application due to their large surface area to volume ratio besides their porous structure. In this work, we produce polymethyl methacrylate (PMMA) nanofibers via electrospinning method in dimethylformamide (DMF) as solvent. Thereafter, we employ a chemical method on final PMMA nanofiberous web to covalently immobilize acetylcholinesterase (AChE) enzyme on membrane surface. Morphology and tensile properties of nanofibers are studied as first steps of characterization to make sure of obtaining a properly stable membrane for enzyme carrying application. Thereafter, the stability and activity of immobilized enzymes as two main characteristic parameters are tested and reported for different applications such as biosensor manufacturing. |
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