扁桃自交不亲和基因的多态性分析

 根据蔷薇科树种S基因的保守序列设计引物, 利用PCR技术对8个新疆扁桃品种S基因进行扩增, 获得了10个大小不同的S基因片段。测序结果表明, 这些DNA片段的核苷酸序列中都具有编码蔷薇科S-RNAase 5个高度保守区域: C1、C2、C3、RC4、C5的序列、编码高变区(RHV) 的序列以及变异度很大的内含子序列, 并且其高变区和内含子序列具有扁桃S基因特异性。同源性分析表明, 获得的10个氨基酸序列与蔷薇科中李亚科S2RNase的氨基酸序列同源性达67% ～96% , 扁桃与樱桃、杏应同属于李亚科。

Analysis on Polymorphism of S Gene in Almond

To investigate the characteristics of the S gene in almond,we degenerative primers according to conservative sequence of S gene in rosaceous plant and got 10 S gene fragments by PCR application from 8 almond cultivars in China.All sequences were aligned with S genes in GeneBank by BLAST software.The result indicated that all 10 fragments contained domain encoding 5 high conservation regions(C1,C2,C3,RC4,C5) and hypervariable region(RHV)of rosaceous S-RNase.RHV and intron characterized S gene furthermore.Homology analysis showed that the amino acid sequence between putative peptides from above S gene fragments and that from S-RNase of rosaceous plant were 67%-96% of homology.Almond should be assigned to plum subspecific groups with cherry and apricot.