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茭白肉质茎膨大过程中保护酶活性变化初探
引用本文:江解增,张强,曹碚生,朱庆森. 茭白肉质茎膨大过程中保护酶活性变化初探[J]. 扬州大学学报(农业与生命科学版), 2004, 25(2): 68-71
作者姓名:江解增  张强  曹碚生  朱庆森
作者单位:扬州大学,农学院,江苏,扬州,225009;扬州大学,农学院,江苏,扬州,225009;扬州大学,农学院,江苏,扬州,225009;扬州大学,农学院,江苏,扬州,225009
基金项目:江苏省“十五”重点攻关项目(BE2001322、BE2002304),江苏省农业三项工程项目[S(01)54]
摘    要:以蒋墅茭为试验材料,于采收盛期选择正常茭不同大小的肉质茎,以雄茭的短缩茎尖为对照,分别测定过氧化物酶(POD)、过氧化氢酶(CAT)、多酚氧化酶(PPO)、苯丙氨酸解氨酶(PAL)以及超氧化物歧化酶(SOD)的活性。结果表明:正常茭白的各种酶在肉质茎的膨大过程中,各种酶活性总体上均呈下降趋势。但POD活性在肉质茎膨大中期有一个上升阶段,PAL、SOD活性在膨大后期低于雄茭,说明POD既参与保护性反应,又参与细胞、组织的分化和细胞膨大。茭白肉质茎的膨大过程是一个感病过程,CAT可能是植物保护性反应的标志酶。

关 键 词:茭白  肉质茎  过氧化物酶  多酚氧化酶  苯丙氨酸解氨酶  超氧化物歧化酶  过氧化氢酶  酶活性
文章编号:1671-4652(2004)02-0068-04
修稿时间:2003-12-12

Primary Study on Changes of Protective Enzymes'Activities in Zizania Gall's Swelling
JIANG Jie-zeng,ZHANG Qiang,CAO Bei-sheng,ZHU Qing-sen. Primary Study on Changes of Protective Enzymes'Activities in Zizania Gall's Swelling[J]. Journal of Yangzhou University(Agricultural and Life Science Edition), 2004, 25(2): 68-71
Authors:JIANG Jie-zeng  ZHANG Qiang  CAO Bei-sheng  ZHU Qing-sen
Abstract:The activities of POD, CAT, PPO, PAL and SOD were investigated in Zizania gall of different swelling stages, using culm's apex of unswollen as control. The results showed that, the activities of different enzymes descended generally in the gall's expanding, but the activities of POD ascended in metaphase of gall's expanding. The activities of PAL and SOD in the gall in anaphase of gall's expanding were lower than that in the apex of unswollen. The results inferred that these enzymes were concerned with protective reactions of plant, also with expand and differentiation of cells and tissues. CAT was also likely to be used as sysbol enzyme of protecive reactions of plant.
Keywords:Zizania latifolia  swollen gall  POD  PPO  PAL  SOD  CAT  enzyme acticities
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