投喂和灌喂裙带菜凝集素对鲫免疫活性物的诱导作用

以裙带菜凝集素(Undaria pinnatifida Lectin)作为免疫增强剂,来研究其对鲫免疫活性的诱导作用。用裙带菜凝集素对鲫(Camssius auratus)进行投喂和灌喂,9d后测定鲫血清的血凝活性、溶菌酶(LSZ)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)的活性与对照组相比都有提高。其中投喂裙带菜凝集素组比投喂对照组依次提高2.0倍,3.1倍,1.2倍,1.7倍;灌喂裙带菜凝集素组比灌喂对照组依次提高4.0倍,3.2倍,1.9倍,1.6倍。这说明凝集素对免疫系统的功能,提高血凝活力、溶菌酶、过氧化氢酶、超氧化物歧化酶活力都有重要作用。由此可以看出来:凝集素可以作为一种免疫增强剂激活鲫的免疫系统,对鲫的非特异性免疫功能具有明显的增强作用。

The induction of Undaria Pinnatifida Lectin to immunity substance in Camssius Auratusby feeding and irrigating

The lectin （UPL） from brown alage Undaria pinnatifida was used immune reinforcer . In this essay we use this lectin to research the inducing affecting of immune activity to Camssius auratus. Then the Camssius auratus were fed using diet containing lectin. The blood clots activity, LSZ activi- ty, CAT activity and SOD activity of blood in Camssius auratus were tested after 9 days,and the re- suits were as follows： When the Camssius auratus were fed with diet containing lectin, their blood clots activity,LAZ activity,CAT activity and SOD activity improved evidently over the control group. The activity of fed lectin from Undaria pinnatifida group was 2.0 , 3.1 , 1.2 and 1.7 times, respec- tively, as much as that of control group . The activity of infusing lectin from Undaria pinnatifida group was 4.0,3.2,1.9 and 1.6 times as much as that of control group. These mean the lectin could enhanced the nonspecific immunization function and it plays a very important role. Thus, we can see the Undaria pinnatifida lectin can be used as immune agent to strength the immune system of Camssius auratus and it can enhance the function of non--specific immunity to crucian obviously.