| 梅花鹿心脏组织cDNA文库的构建 |
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| 引用本文： | 幺宝金,赵雨,李娟,牛放,杨菲. 梅花鹿心脏组织cDNA文库的构建[J]. 安徽农业科学, 2011, 39(1): 277-278,281 |
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| 作者姓名： | 幺宝金 赵雨 李娟 牛放 杨菲 |
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| 作者单位： | 吉林大学公共卫生学院;长春中医药大学中医药与生物工程研发中心;长春中药大学中医药与生物工程研发中心; |
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| 基金项目： | 国家“十一五”科技支撑项目(2007BA138B06); 吉林省科技发展计划项目(20096042) |
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| 摘 要： | [目的]构建梅花鹿心脏cDNA文库。[方法]提取梅花鹿心脏总RNA,纯化mRNA;合成双链cDNA,连接载体,并电转化至感受态细胞;测定文库的克隆数、重组率及插入片段大小。[结果]经鉴定,所构建梅花鹿心脏组织cDNA文库,库容量为3.8×106个克隆,重组率为100%,插入片段大小为0.5～2.0kb,平均长度约为1.0kb。[结论]所构建cDNA文库的各项指标均达到要求。
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| 关 键 词： | 梅花鹿 心脏 cDNA文库 |
Construction of cDNA Library from the Heart of Sika Deer |
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| YAO Bao-jin et al. Construction of cDNA Library from the Heart of Sika Deer[J]. Journal of Anhui Agricultural Sciences, 2011, 39(1): 277-278,281 |
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| Authors: | YAO Bao-jin et al |
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| Affiliation: | YAO Bao-jin et al (School of Public Health,Jilin University,Changchun,Jilin 130021) |
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| Abstract: | [Objective]To construct a cDNA library from the heart of sika deer.[Method]Total RNA was extracted from the heart of sika deer,and mRNA was purified.Double-stranded cDNA was synthesized,then connected with vector and electrotransformated to competent cells.The number of clones,recombinant rate and the length of insert fragments were checked.[Result]The capacity of the cDNA library was 3.8×106 with a recombinant rate of 100%.The length of insert fragments ranged from 0.5 kb to 2.0 kb,and the average length was about 1.0 kb.[Conclusion] The cDNA library constructed was qualified. |
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| Keywords: | Sika deer Heart cDNA library |
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