| mRNA差异显示PCR的研究进展 |
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| 引用本文： | 安健,汪明,王黎霞,杨静,夏茂华. mRNA差异显示PCR的研究进展[J]. 北京农学院学报, 2005, 20(2): 64-68 |
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| 作者姓名： | 安健 汪明 王黎霞 杨静 夏茂华 |
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| 作者单位： | 北京农学院动物科学技术系,北京,102206;中国农业大学动物医学院,北京,100094;北京动物园,北京,100044 |
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| 基金项目： | 国家科技攻关项目 , 北京市自然科学基金 , 北京市委组织部优秀人才基金 |
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| 摘 要： | mRNA差异显示PCR技术是在基因转录水平上研究差异表达和性状差异的有效方法之一,该方法在生物的发育、性状和对各种生物、理化因子作用时应答过程基因表达的研究中应用十分广泛,笔者简述了mRNA差异显示PCR技术的基本原理、技术改进以及此技术在球虫抗药性研究中的应用。
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| 关 键 词： | mRNA差异显示PCR 球虫 抗药性 |
| 文章编号： | 1002-3186(2005)02-0064-05 |
| 修稿时间： | 2004-04-10 |
mRNA Differential Display and Its Application on Drug-Resistance of Coccidia |
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| AN Jian,WANG Ming,WANG Li-xia,YANG Jing,XIA Mao-hua. mRNA Differential Display and Its Application on Drug-Resistance of Coccidia[J]. Journal of Beijing Agricultural College, 2005, 20(2): 64-68 |
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| Authors: | AN Jian WANG Ming WANG Li-xia YANG Jing XIA Mao-hua |
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| Abstract: | The mRNA differential display is a new technique to clone genes of differential expressing of the diversity organism. The technique is widely used in the diversity organism on the development cell division, differentiation, metabolism and the response to the pathogen. The mechanism, the method, the development and improvement of the application of the technique are also reviewed and prospected in the paper. The application of the technique in Coccidian drug-resistance is introduced. |
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| Keywords: | mRNA differential display PCR Coccidia drug-resistance |
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