| 家榆种子人工老化过程中的细胞形态学特征 |
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| 引用本文： | 胡蝶,王琼,刘雪萍,汪晓峰. 家榆种子人工老化过程中的细胞形态学特征[J]. 北京林业大学学报, 2010, 32(6): 1-8 |
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| 作者姓名： | 胡蝶 王琼 刘雪萍 汪晓峰 |
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| 作者单位： | 林木育种国家工程实验室，林木花卉遗传育种教育部重点实验室，国家林业局树木花卉育种与生物工程重点开放实验室，北京林业大学生物科学与技术学院 |
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| 基金项目： | 国家自然科学基金项目(30570178) |
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| 摘 要： | 以家榆种子为材料,在37℃、100%相对湿度下进行人工老化。应用电子显微镜、光学显微镜观察、细胞原位末端脱氧核苷酸转移酶标记(TUNEL)法检测家榆种子在人工老化条件下细胞发生凋亡的形态学特征。结果显示:老化第1天根冠少数几个细胞开始出现细胞凋亡;随着老化深入,生长点、子叶等多个部位开始有凋亡细胞出现;老化至第6天,部分凋亡细胞崩解,细胞质高度浓缩,剩余组织细胞膜的保护能力下降,出现真菌感染;同时,用外源Ca2+作为诱导剂对家榆种子进行处理,TUNEL法检测结果显示并未出现细胞凋亡。
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| 关 键 词： | 家榆种子 细胞凋亡 TUNEL检测法 人工老化 |
| 收稿时间： | 1900-01-01 |
Morphological characteristics of Ulmus pumila L.seeds during artificial aging |
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| HU Die,WANG Qiong,LIU Xue-ping,WANG Xiao-feng. Morphological characteristics of Ulmus pumila L.seeds during artificial aging[J]. Journal of Beijing Forestry University, 2010, 32(6): 1-8 |
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| Authors: | HU Die WANG Qiong LIU Xue-ping WANG Xiao-feng |
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| Affiliation: | National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest and Ornamental Plants of Ministry of Education, Tree and Ornamental Plant Breeding and Biotechnology Laboratory of State Forestry Administration,College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, 100083, P.R. China. |
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| Abstract: | In this study, morphological characteristics of Ulmus pumila seeds during artificial aging was studied. The seeds were treated at a high temperature of 37℃ and high humidity (100% relative umidity). U. pumila L. seeds during artificial aging were examined using electron microscope, optical microscope and TUNEL method. The results showed that a few TUNEL positive cells were found in the root cap (RC) on the first day of artificial aging. Then TUNEL positive cells gradually appeared at shoot apex (SA), cotyledons (C) and so on during the next few days. Until the sixth day of aging time some cells were disintegrated, highly condensed cytoplasm appeared, membranes lost their protective capability and were found to be susceptible to epiphyte. We also used CaCl2 to treat Elm seeds, but TUNEL positive cells were not found. |
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| Keywords: | Ulmus pumila seeds programmed cell death (PCD) TUNEL method artificial aging |
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