| 二级竞争酶联免疫孕酮临界点判别分析在乳牛早期妊娠诊断上的应用 |
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| 引用本文： | 龚振明,耿世祥,陈鲁勇. 二级竞争酶联免疫孕酮临界点判别分析在乳牛早期妊娠诊断上的应用[J]. 上海交通大学学报(农业科学版), 1996, 0(3) |
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| 作者姓名： | 龚振明 耿世祥 陈鲁勇 |
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| 作者单位： | 上海农学院动物科学系 |
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| 摘 要： | 本文根据二级竞争酶联免疫定量测定的原理建立了用于乳牛早期妊娠诊断的二级竞争酶联免疫孕酮临界点判别分析法．本方法无需仪器比色和数学计算，反应结果清晰可辨、操作简便．根据对５２０头次配种后２１ｄ乳牛进行测试，其总正确率为８３．８％，其中阴性正确率为９０％，阳性正确率为７９％。
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| 关 键 词： | 二级竞争；酶联免疫；孕酮；乳牛；妊娠诊断 |
EARLY PREGNANCY DIAGNOSIS OF COWS WITH DOUBLE COMPETITION EIA OF PROGESTERONE MEASUREMENT |
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| Gong Zhenming,Geng Shixiang,Chen Luyong. EARLY PREGNANCY DIAGNOSIS OF COWS WITH DOUBLE COMPETITION EIA OF PROGESTERONE MEASUREMENT[J]. Journal of Shanghai Jiaotong University (Agricultural Science), 1996, 0(3) |
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| Authors: | Gong Zhenming Geng Shixiang Chen Luyong |
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| Abstract: | A double competition EIA was developed to measure progesterone for early pregnancy disgnosis of cows. By direct observing the result of reaction, the accurracy in assessment of pregnancy status by measurement of progesterone in skim milk of 520 cows collected at 21 days after artificial insemination,which was later confirmed by rectal palpation, was 79% for pregnancy and 90% for nonpregnancy. |
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| Keywords: | Double competition Enzyme immunoassay Progesterone Cow Pregnancy diagnosis |
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