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| 郑58和掖478玉米自交系基因组差异性分析 | |
| 引用本文： | 王彦玲,卫文星,铁双贵,王延召,朱卫红,岳润清,齐建双.郑58和掖478玉米自交系基因组差异性分析[J].玉米科学,2010,18(3):57-60. |
| 作者姓名： | 王彦玲 卫文星 铁双贵 王延召 朱卫红 岳润清 齐建双 |
| 作者单位： | 1. 郑州大学生物工程系,郑州,450001;河南省农业科学院粮食作物研究所,郑州,450002 2. 河南省玉米生物学重点实验室,郑州,450002 3. 河南省玉米生物学重点实验室,郑州,450002;河南省农业科学院粮食作物研究所,郑州,450002 |
| 基金项目： | 国家自然科学基金项目“玉米特用品质基因在我国骨干种质遗传背景中的互作效应及应用潜势研究”(30871539) |
| 摘 要： | 采用SSR标记对我国核心种质郑58和掖478进行基因组差异性分析。结果表明:200对引物中有57对引物具有多态性,多态性标记比率达到28.5%,遗传相似度为71.5%。200对引物共计扩增出424个SSR等位性片段,其中有113个多态性等位片段,遗传杂合度为26.7%,遗传相似度为74.3%。SSR多态性标记在不同染色体上呈现不均匀性分布,在同一染色体上不同区域分布密度不同,具有多态性标记集中区域,基因组差异可能与自交系配合力变化有关。 |
| 关 键 词： | 玉米 SSR标记 DNA 基因组差异 |
| 收稿时间： | 2009/12/10 0:00:00 |
Analysis of Genomes Difference Between Zheng58 and Ye478 |
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| WANG Yan-ling,WEI Wen-xing,TIE Shuang-gui,et al..Analysis of Genomes Difference Between Zheng58 and Ye478[J].Journal of Maize Sciences,2010,18(3):57-60. | |
| Authors: | WANG Yan-ling WEI Wen-xing TIE Shuang-gui |
| Abstract: | The genome difference between the core collection of Zheng58 and Ye478 was studied using 200 SSR markers in this paper.The results showed that 57 SSR primers were polymorphic,the polymorphism percentage was 28.5 and the genetic relatedness was 71.5 .Four hundred and twenty four amplified fragments and 113 polymorphic fragments of 200 SSR primers were detected.The heterozygosis was 26.7 and the genetic relatedness was 74.3 .SSR markers in different chromosomes and them from different bins of same chromosome appeared uniformly,possessing concentrated region.Genome difference may be related with combining ability variation of inbred lines. |
| Keywords: | DNA |
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