| 野桑蚕CYP305BlVl基因的组织表达特异性初探 |
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| 引用本文： | 陈玉华 卫正国 王东 赵华强 贡成良 沈卫德. 野桑蚕CYP305BlVl基因的组织表达特异性初探[J]. 江苏蚕业, 2005, 27(4): 11-13 |
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| 作者姓名： | 陈玉华 卫正国 王东 赵华强 贡成良 沈卫德 |
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| 作者单位： | 苏州大学蚕桑研究所、苏州大学生命科学学院生物资源与环境科学系,215123 |
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| 基金项目： | 国家973项目（No：2005CB121000）、国家自然科学基金（No：30471309） |
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| 摘 要： | 抽提不同处理的野桑蚕各组织总mRNA，利用地高辛标记的野桑蚕CYP305BlVl的cDNA基因作为探针，通过点杂交方法研究野桑蚕CYP305lBl基因的组织表达特异性。实验结果显示，CYP305BlVl基因在氟化物诱导后的野桑蚕中肠、脂肪体、马氏管、体壁中有特异性转录。
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| 关 键 词： | CYP305BlVl基因 探针 点杂交 组织表达 定位 |
Preliminary research of tissue-specific expression of CYP305BlVl from Bombyx mandarina |
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| Chen Yuhua, Wei Zhengguo, Wang Dong, Zhao Huaqiang, Gong Chengliang, Shen Weide. Preliminary research of tissue-specific expression of CYP305BlVl from Bombyx mandarina[J]. Jiangsu Sericulture, 2005, 27(4): 11-13 |
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| Authors: | Chen Yuhua Wei Zhengguo Wang Dong Zhao Huaqiang Gong Chengliang Shen Weide |
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| Affiliation: | Institute of Sericuhure, Suzhou University ; Department of Biological Resources and Environmental Science, School of Life Sciences, Suzhou University |
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| Abstract: | Total mRNA was extracted from different tissues of Bombyx mandarina induced by sodium fluoride and was hybridized with digoxin - tagged CYP305BlVl cDNA by dot blot to research the tissue-specific expression of CYP305BlVl gene from Bombyx mandarina. The results showed that CYP305BlVl gene was expressed in midgut, fat body, Malpighian tube, and body wall. |
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| Keywords: | CYP305BlVl Probe Dot blot Tissue-specific expression Localization |
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