鼓粒期淹水胁迫对大豆叶片AsA-GSH循环的损伤及烯效唑的缓解效应

为研究淹水胁迫下喷施烯效唑(S3307)对不同大豆品种的影响,以耐涝品种'垦丰14'和涝渍敏感品种'垦丰16'大豆为试验材料,进行盆栽试验,研究鼓粒期(R5)淹水胁迫对大豆叶片膜脂过氧化程度(MDA)、活性氧(ROS)和抗坏血酸-谷胱甘肽(AsA-GSH)循环系统的损伤及S3307的缓解效应.结果表明:R5期淹水胁迫显...

Damage to the AsA-GSH cycle of soybean leaves under waterlogging stress at in seed filling period growth stages and the mitigation effect of uniconazole

This research explored the effect of spraying uniconazole （S3307） on two different soybean varieties under waterlogging stress. The waterlogging-tolerant variety Kenfeng 14 and the waterlogging-sensitive variety Kenfeng 16 were studied in pot experiments. The degree of membrane lipid peroxidation as indicated by malondialdehyde （MDA） concentration， and levels of reactive oxygen species （ROS） and peptide ascorbic acid-glutathione （AsA-GSH） in soybean leaves under waterlogging stress at growth stage R5 （seed filling period） were evaluated. AsA-GSH circulatory system damage was detected and S3307 had an alleviating effect. The results showed that waterlogging stress at the R5 growth stage significantly increased the MDA content in the leaves of the two tested soybean varieties and accelerated the accumulation of ROS. A greater increase was observed in the waterlogging-sensitive variety Kenfeng 16 than in Kenfeng 14. After foliar spraying with S3307， the concentration of non-enzyme antioxidants and the activities of key enzymes were increased， and the MDA concentration of leaves was reduced. S3307 inhibited the accumulation of ROS， reduced the damage caused by waterlogging stress to the membrane system， and assisted the maintenance of a high level of key enzyme activity and non-enzymatic antioxidant concentrations. The benefits of S3307 application persisted after plants were returned to normal soil water levels， S3307 and promoted the recovery of the two soybean varieties to a normal growth status. The recovery ability of waterlogging-tolerant variety Kenfeng 14 was better than Kenfeng 16. In summary， waterlogging stress had contrast effects on the AsA-GSH cycle in the leaves of two soybean varieties with different waterlogging tolerances. S3307 partially alleviated the damage caused by the waterlogging stress.