茯砖茶对葡聚糖硫酸钠诱导UC小鼠炎症及肠道微生物的影响

为研究茯砖茶对葡聚糖硫酸钠(Dextran sulfate sodium,DSS)诱导的溃疡性结肠炎(Ulcerative colitis,UC)小鼠的抗炎作用及肠道微生物多样性的影响,将60只C57BL/6雌性小鼠随机分成正常对照组、10 mg·kg-1 EGCG对照组、高剂量茯砖茶(300 mg·kg-1)对照组、...

Effects of Fu Brick Tea on Inflammation and Intestinal Microflora Diversity in Mice with DSS-induced Ulcerative Colitis

In this study, we investigated the effects of Fu brick tea on the anti-inflammation and intestinal microflora of ulcerative colitis (UC) mice induced by Dextran sulfate sodium (DSS). Sixty C57BL/6 female mice were randomly divided into the normal control group, 10 mg·kg-1 EGCG control group, high dose brick tea (300 mg·kg-1, HBT) control group, DSS model group, DSS＋10 mg·kg-1 EGCG group, DSS＋low dose brick tea (100 mg·kg-1, LBT) group, DSS＋middle dose brick tea (150 mg·kg-1, MBT) group and DSS＋HBT group, with the control groups n=5 and the treatment groups n=9. After 1 week of DSS modeling, the mice were gavaged for 4 weeks. The mice were executed after 5 weeks of the treatments. The histopathological changes of mice colon were observed, and the disease activity index (DAI) of mice colitis was assessed. The levels of IL-6, IL-8, IL-1β, TNF-α and IFN-γ inflammatory factors in mice serum and the expression levels of JAK2 and STAT3 genes in mice colon tissues were measured. The intestinal microflora of mice was analyzed by PCR-DGGE and clone sequencing techniques. The results show that compared with the DSS model group, the quality of survival and colonic tissue morphology of mice were significantly improved after feeding EGCG and different concentrations of brick tea, and the levels of inflammatory factors IL-6, IL-8, IL-1β, TNF-α and IFN-γ in mice serum were significantly reduced (P<0.05). Moreover, the expressions of JAK2 and STAT3 genes were significantly reduced (P<0.05) JAK2/STAT3 inflammatory signaling pathway was inhibited; and intestinal microflora diversity and richness were increased significantly. The dominant bacterial flora in different treatment groups were changed as well. In conclusion, Fu brick tea can ameliorate DSS-induced UC injury by inhibiting JAK2/STAT3 inflammatory signaling pathway and modulating intestinal microflora diversity.