| 香花槐花器官的组织培养及植株再生研究 |
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| 引用本文： | 王玲仙,谢吉容,付坚,殷富有,黄兴奇. 香花槐花器官的组织培养及植株再生研究[J]. 北方园艺, 2008, 0(1): 191-193 |
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| 作者姓名： | 王玲仙 谢吉容 付坚 殷富有 黄兴奇 |
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| 作者单位： | 1. 云南省农业科学院,生物技术与种质资源研究所,云南,昆明,650223
2. 重庆文理学院,生命科学系,四川,重庆,402168 |
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| 基金项目： | 云南省农业科技攻关资助项目 |
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| 摘 要： | 采用香花槐的花器官做外植体,进行组织培养和植株再生系统的研究.结果表明:2,4-D对于香花槐愈伤诱导的启动是必需的,而多种激素配比会使诱导率提高;选择浓度为0.3～0.5 mg/L的BA和浓度为0.1 mg/L的NAA配合使用,能使花药愈伤达到较高分化率;1/2MS IBA 0.05 mg/L和1/2MS IBA 1.0 mg/L的诱导组培苗生根效果较好.
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| 关 键 词： | 香花槐 花药培养 再生植株 愈伤组织 |
| 文章编号： | 1001-0009（2008）01-0191-03 |
| 收稿时间： | 2007-08-23 |
| 修稿时间： | 2007-08-23 |
Study on Flower Tissue Culture and Plant Regeneration of Robinia idaho |
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| WANG Ling-Xian,XIE Ji-rong,FU Jian,YIN Fu-you,HUANG Xing-Qi. Study on Flower Tissue Culture and Plant Regeneration of Robinia idaho[J]. Northern Horticulture, 2008, 0(1): 191-193 |
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| Authors: | WANG Ling-Xian XIE Ji-rong FU Jian YIN Fu-you HUANG Xing-Qi |
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| Abstract: | Robinia idaho regeneration system and its tissue culture has been studied with explant of different part of its flower.The results show that 2,4-D is indispensable for callus induction of anther,ovary and filament while receptacles and petals cannot be induced in this experiment.Combination of 0.3-0.5 mg/L BA and 0.1 mg/L NAA are effective to differentiate anther callus.1/2MS medium supplemented with IBA 0.05mg/L and 1/2MS IBA 1.0mg/L can well promote seedings to take roots. |
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| Keywords: | Robinia idaho Anther culture Plant regeneration Ballus introduction |
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