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小麦RIL群体SSR标记偏分离的遗传分析
引用本文:刘刚,许盛宝,倪中福,李晶,秦丹丹,窦秉德,彭惠茹,孙其信. 小麦RIL群体SSR标记偏分离的遗传分析[J]. 农业生物技术学报, 2007, 15(5): 828-833
作者姓名:刘刚  许盛宝  倪中福  李晶  秦丹丹  窦秉德  彭惠茹  孙其信
作者单位:1. 新疆农业大学农学院,乌鲁木齐,830052
2. 中国农业大学植物遗传育种系,北京,100094
3. 淮阴师范学院植物生物技术研究所,淮安,223300
基金项目:国家重点基础研究发展计划(973计划)
摘    要:以普通小麦3338与斯卑尔脱小麦Altgold杂交得到的F6代重组近交系群体为材料,筛选出334个(271个SSR和63个EST-SSR标记)多态性标记,以此为基础构建了一个包含287个分子标记的遗传图谱。对这些多态性标记进行偏分离分析发现82个表现为偏分离(P<0.05),其中70个可以定位到遗传连锁图谱中。这些偏分离标记中有33个标记位点偏向母本3338,占40.2%,49个标记位点偏向父本Altgold,占59.8%。这些偏分离标记在图谱上的分布有两种:成簇分布和孤立位点的偏分离。在5条不同染色体上发现6个偏分离热点区域,这些偏分离热点区域的形成可能与配子体选择有关。

关 键 词:小麦  偏分离  分子标记  重组近交系
文章编号:1006-1304(2007)05-0828-06
收稿时间:2007-03-05
修稿时间:2007-04-02

Genetic Analysis of Segregation Distortion of Molecular Markers in Wheat RIL Population
LIU Gang,XU Sheng-bao,NI Zhong-fu,LI Jing,QIN Dan-dan,DOU Bing-de,PENG Hui-ru,SUN Qi-xin. Genetic Analysis of Segregation Distortion of Molecular Markers in Wheat RIL Population[J]. Journal of Agricultural Biotechnology, 2007, 15(5): 828-833
Authors:LIU Gang  XU Sheng-bao  NI Zhong-fu  LI Jing  QIN Dan-dan  DOU Bing-de  PENG Hui-ru  SUN Qi-xin
Affiliation:1.College of Agriculture, Xinjiang Agricultural University, Urumqi 830052, China; 2.Department of Plant Breeding, China Agricultural University, Beijing 100094, China; 3.Institute of Plant Biotechnology, Huaiyin Teachers College, Huaian 223300, China
Abstract:Abstract: Using a RIL population derived from a cross between a common wheat 3338 and spelt wheat Altgold, a genetic linkage map was constructed comprising of 287 out of totally 344 polymorphic markers (271 SSR and 63 EST-SSR markers). Among the 334 polymorphic markers, 82 markers showed the significantly segregation distortion (P<0.05), favoring either the maker alleles of female parent 3338(33, 40.2%) or male parent Altgold (49,59.8%). Segregation distortion markers distribution along the present molecular maps of wheat was far from uniform, with clusters of tightly linked loci and single markers. 6 segregation distortion regions (clusters of 3-6 makers) were detected on 5 chromosomes, indicating that possible causes for segregation deviation of molecular markers are gametic selection.
Keywords:wheat  segregation distortion  molecular marker  RIL
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