Mapping B-cell linear epitopes of NS3 protein of bovine viral diarrhea virus |
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Authors: | Yan Li Ying Jia Kai Wen Hua Liu Mingchun Gao Bo Ma Wenlong Zhang Junwei Wang |
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Institution: | College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang, 150030, PR China |
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Abstract: | Bovine viral diarrhea virus (BVDV) is a member of the genus Pestivirus within the family Flaviviridae. NS3 is one of the immunodominance regions of the BVDV viral proteins. To identify the potential B-cell linear antigenic epitopes within BVDV NS3 region, serial overlapping truncations covering the whole region were expressed and purified, and screened by multistep of Western-blot. We found (1VCKKITEHERCHVNI15), (20AFFGVMPRGTTPRAPVR36), (46RRGLETGWAYTHQGGI61), (281EGDMATGITYASYGYFC297), (426YSGEDPANLRVVTSQSPYVVVATNAIESGV455) and (481FIVTGLKRMAVTVGEQA497) can be recognized by the BVDV infected bovine serum. These proteins have been confirmed by indirect enzyme-linked immunosorbent assay (I-ELISA). The results of this study might open new perspectives on the structure and antibody–antigen reaction of the non-structural proteins and may aid in the clinical application as well. |
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