| 猪带绦虫囊尾蚴病特异性抗原筛选的研究进展 |
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| 引用本文： | 庞建达,王海英,杜立银,孙树民. 猪带绦虫囊尾蚴病特异性抗原筛选的研究进展[J]. 中国农学通报, 2017, 33(11): 131-136 |
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| 作者姓名： | 庞建达 王海英 杜立银 孙树民 |
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| 作者单位： | 内蒙古民族大学动物科学技术学院,内蒙古通辽市科尔沁区兽医局,内蒙古民族大学动物科学技术学院,内蒙古民族大学动物科学技术学院 |
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| 基金项目： | 国家自然科学基金项目“猪带绦虫/囊尾蚴不同发育时期标识性抗原基因的筛选”(31460658)，“我国旋毛虫属分类鉴定和分子进化研究”(31160504)；中国博士后科学基金项目“旋毛虫引发宿主高度免疫抑制的分子机制研究”(2012M520674)。 |
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| 摘 要： | 旨在为猪带绦虫囊尾蚴病免疫诊断方法和有效防治措施的建立奠定理论基础。阐述了猪带绦虫囊尾蚴病流行与危害及诊断和防控中存在的问题，回顾了利用cDNA文库筛选、噬菌体展示文库筛选、噬菌体肽库筛选技术和双向电泳免疫印迹筛选等几种技术对猪带绦虫囊尾蚴病特异性抗原的筛选，并将筛选到的特异性抗原进行简要介绍，分别阐明各抗原组分在该病诊断、预防和致病机理中作用及可能潜在的价值。通过猪带绦虫囊尾蚴病特异性抗原筛选说明，开展该病大规模、无渗漏抗原筛选研究，是解决虫体感染过程中不同发育时期免疫诊断、疫苗研制问题的关键。其研究重要性不但对该病本身的诊断和防控具有重要的意义，同时也为寄生虫病的诊断和防控提供新的思路。
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| 关 键 词： | 猪带绦虫；囊尾蚴；特异性抗原；抗原筛选 |
| 收稿时间： | 2016-06-17 |
| 修稿时间： | 2016-10-25 |
Research Progress of Specific Antigen Screening for Taeniasis/Cysticercosis |
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| Pang Jiand,Wang Haiying,Du Liyin and Sun Shumin. Research Progress of Specific Antigen Screening for Taeniasis/Cysticercosis[J]. Chinese Agricultural Science Bulletin, 2017, 33(11): 131-136 |
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| Authors: | Pang Jiand Wang Haiying Du Liyin Sun Shumin |
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| Affiliation: | College of Animal Science and Technology, Inner Mongolia University for Nationalities, Tongliao Inner Mongolia,Veterinary Department of Horqin, Tongliao Inner Mongolia ),College of Animal Science and Technology, Inner Mongolia University for Nationalities, Tongliao Inner Mongolia,College of Animal Science and Technology, Inner Mongolia University for Nationalities, Tongliao Inner Mongolia |
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| Abstract: | The study aims to provide theoretical bases for the establishment of immunodiagnosis methods and effective control measures of taeniasis/cysticercosis. We first expounded the prevalence, harm and the problems in the diagnosis, prevention and control of taeniasis/cysticercosis. Then we reviewed the technologies for screening specific antigens of taeniasis/cysticercosis, including cDNA library, phage display library, phage display peptide library and two dimensional PAGE western blotting, briefly described the obtained specific antigen, and expounded potential value of the antigens in diagnosis, prevention and pathogenesis of the disease. Specific antigen screening of taeniasis/cysticercosis provide that it is key to develop the largescale, no leakage antigen screening studies of the disease for solving the problems of immune diagnosis in the different development stages of worm infection and developing vaccine.The study, being important to the diagnosis, prevention and control of taeniasis/cysticercosis, also provides a reference for the diagnosis, prevention and control of other parasitic diseases. |
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| Keywords: | Taenia solium cysticercus cellulosae specific antigen antigen Screening |
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