中间锦鸡儿生长发育过程中5个miRNAs及其靶基因的表达模式分析

利用qRT-PCR技术分析了5个miRNAs(cin-miR157a、cin-miR159a、cin-miR165a、cin-miR172b和cin-miR396a)及其靶基因在中间锦鸡儿(Caragana intermedia)不同发育阶段不同器官的表达变化。结果显示,5个miRNAs的表达模式均存在时空特异性,其中cin-miR159a、cin-miR165a、cin-miR172b、cin-miR396a在成年期营养器官中强烈表达,在幼年期和成年期生殖器官中弱表达;cin-miR157a在幼年期和成年期生殖器官中强烈表达,在成年期营养器官中弱表达。除cin-miR159a的靶基因MYB和cin-miR165a的靶基因HD-Zip Ⅲ外,其它被检测的miRNAs靶基因与相应的miRNAs的表达呈现出明显的相互消长的表达模式。该结果表明中间锦鸡儿miRNA可通过调控其靶基因的表达,进而调控发育阶段转变、器官形态建成、细胞分化等生物学过程。研究结果将为中间锦鸡儿的定向改良工作提供理论依据和基因资源。

Expression Analysis of 5 miRNAs and Their Targets During Different Growth and Developmental Stages of Caragana intermedia

The expression patterns of 5 miRNAs(cin-miR157a, cin-miR159a, cin-miR165a, cin-miR172b and cin-miR396a) and their targets during different organs in different developmental stages were assessed by qRT-PCR. The results showed that the expression patterns of all the miRNAs are of temporal and spatial specificity. 4 miRNAs(cin-miR159a, cin-miR165a, cin-miR172b and cin-miR396a) are highly expressed in vegetative organs of mature period; one miRNA (cin-miR157a) is highly expressed in juvenile stage and reproduction organs of mature period. The results also showed that three miRNAs (cin-miR157a, cin-miR172b and cin-miR396a) and their target genes were related to the growth and decline in different developmental stages and different organs. High expression of miRNA and its target gene expression are often low. When the miRNA expression is low, its target gene expression is often high. This result indicates that miRNA may play an important role during the growth and development of C. intermedia.